Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,758 results for: nif

Showing: 1281 - 1300 of 1758 results

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D. vulgaris Cas7/pHMGWA

Plasmid

#81184

Purpose

Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1072A

Plasmid

#91859

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R1072A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

R1072A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1082A

Plasmid

#91863

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1082A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1082A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTet_KaeCanABC(G189K)

Plasmid

#248957

Purpose

CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G189K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

KaeCanABC(G189K)

Expression

Bacterial

Mutation

G189K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_KaeCanABC(G104K,G189K)

Plasmid

#248958

Purpose

CanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

KaeCanABC(G104K,G189K)

Expression

Bacterial

Mutation

G104K,G189K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLG017_Avs1a(I134K,I234K)

Plasmid

#248947

Purpose

AVAST from E. piriflorinigrans with Avs1a mutated (I134K, I234K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Avs1a(I134K,I234K)

Expression

Bacterial

Mutation

I134K,I234K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_KaeCanA(C187A)BC

Plasmid

#248954

Purpose

CanABC from K. aerogenes under control of pTet and the native promoter, with CanA mutated (C187A)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

KaeCanA(C187A)BC

Expression

Bacterial

Mutation

C187A

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMAB(D207A)

Plasmid

#248934

Purpose

Hachiman from P. fluorescens under pTet control with HamB mutated (D207A)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMAB(D207A)

Expression

Bacterial

Mutation

D207A

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLG017_FLAG-Avs1a(I134K,I234K)

Plasmid

#248949

Purpose

AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

FLAG-Avs1a(I134K,I234K)

Tags

3xFLAG

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamM(I118K)AB

Plasmid

#248935

Purpose

Hachiman from P. fluorescens under pTet control with HamM mutated (I118K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamM(I118K)AB

Expression

Bacterial

Mutation

I118K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_KaeCanABC(G104K)

Plasmid

#248956

Purpose

CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G104K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

KaeCanABC(G104K)

Expression

Bacterial

Mutation

G104K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamM(I118K,I218K)AB

Plasmid

#248937

Purpose

Hachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamM(I118K,I218K)AB

Expression

Bacterial

Mutation

I118K,I218K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(deltaA)B

Plasmid

#248938

Purpose

Hachiman from P. fluorescens under pTet control with the dHamA domain removed

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(deltaA)B

Expression

Bacterial

Mutation

HamA domain deletion

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_KaeCanABC(H271A)

Plasmid

#248955

Purpose

CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (H271A)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

KaeCanABC(H271A)

Expression

Bacterial

Mutation

H271A

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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exp_KaeCanAB

Plasmid

#248959

Purpose

His-MBP-CanAB from K. aerogenes for overexpression.

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Kae(10xHis-MBP-TEV-)CanAB

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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exp_PflHamM

Plasmid

#248943

Purpose

His-MBP-HamM from P. fluorescens for overexpression.

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Pfl(10xHis-MBP-TEV-)HamM

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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exp_KaeCanC

Plasmid

#248960

Purpose

His-MBP-CanC from K. aerogenes for overexpression.

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Kae(10xHis-MBP-TEV-)CanC

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(S157A)B

Plasmid

#248933

Purpose

Hachiman from P. fluorescens under pTet control with HamA mutated (S157A)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(S157A)B

Expression

Bacterial

Mutation

S157A

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1281 - 1300 of 1758 results

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