We narrowed to 4,090 results for: PRS

1. pRS314-prp16-302

   Plasmid

   #89974

   Purpose

   prp16-302 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-302 allele (R456K and G691R)

   Promoter

   Available since

   Aug. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS303 prp8-101

   Plasmid

   #111307

   Purpose

   prp8-101

   Depositor

   Christine Guthrie

   Article

   Umen et al Genetics. 1996 Jun;143(2):723-39.

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp8-101 (E1960K)

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS316 GAL cluster

   Plasmid

   #61925

   Purpose

   Expression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316

   Depositor

   Jon Houseley

   Article

   Cruz et al Elife. 2014 Feb 11;3:e01581. doi: 10.755

   Inserts

   GAL1 (GAL1 Budding Yeast)
   GAL10 (GAL10 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET FLII275Pglu-4.6m

   Plasmid

   #13565

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A G275S-ECFP-K276R
   

   Use

   Tags

   Citrine

   Expression

   Bacterial

   Mutation

   F16A, ECFP insert in mglB

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET FLIPE-10u

   Plasmid

   #13538

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET a sfGCaMP6s-T78H

   Plasmid

   #100023

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s-T78H
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP and T78H m…

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSETa-mEos4b-V69T

   Plasmid

   #98572

   Purpose

   photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos4b-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET FLIPglu-2uDelta13

   Plasmid

   #13561

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F D152A Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A, D152A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS424-TUB1-Cterminal6xHis

    Plasmid

    #60387

    Purpose

    Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    6xHIS

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only