We narrowed to 2,414 results for: organization Addgene

1. miniSOG-ER-5

   Plasmid

   #57766

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   ER (CALR Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pQ54-VC

   Plasmid

   #175060

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ54 (HTT Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQ25-VC

   Plasmid

   #175062

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ25 (HTT Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQ39-VC

   Plasmid

   #175061

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ39 (HTT Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-AKAP95-FRB

   Plasmid

   #138254

   Purpose

   Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   AKAP95-FRB (AKAP8 Human)
   

   Use

   Tags

   FRB rapamycin binding domain and HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

   Plasmid

   #112837

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SH2 + SH3 (C-term) domains of Grb2

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniSOG-Mito-7

   Plasmid

   #57773

   Purpose

   Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 8xNFAT-ZsG-hCD4

   Plasmid

   #162746

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5 Myc-tagged PSK

   Plasmid

   #197149

   Purpose

   Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

   Insert

   TAOK2 (TAOK2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   SV40

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Expression

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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