2,223 results

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#227773

Purpose

Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and tp1

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#226834

Purpose

Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

Olactb and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTol1 - 14xUAS:CaSR-EGFP

Plasmid

#191502

Purpose

A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol1 transgenes…

Promoter

14X UAS

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-hcar1-4-mKate2

Plasmid

#226712

Purpose

overexpression red fluorescent hcar1-4 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

hcar1-4

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-5.0dand5:EGFP

Plasmid

#223327

Purpose

Expresses EGFP in Kupffer's vesicle cells in zebrafish embryos

Depositor

Hiroyuki Takeda

Article

Ikeda et al Dev Growth Differ. 2022 Apr;64(3):163-17

Insert

5.0kb dand5 promoter (dand5 Zebrafish)

Use

Zebrafish expression

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-dr_Unc (JDW 1365)

Plasmid

#224482

Purpose

Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Unc-503 promoter

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2

Plasmid

#218208

Purpose

Zebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-2.1prox1a_2

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2_-87prox1a:basEGFP_Acry:GFP

Plasmid

#218213

Purpose

Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-87prox1a

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDR274-gsc2-sgRNA

Plasmid

#184818

Purpose

Used to synthesize gRNA targeting exon 2 of the gsc2 gene

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

gsc2-sgRNA

Use

CRISPR; Template for sgrna synthesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP-rln3a-HA

Plasmid

#184820

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

rln3a-HA

Use

CRISPR; For integration of qf2 by homology-direct…

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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