We narrowed to 15,962 results for: GRN

1. pU6-Sp-gRNA-ALB_DF_A277c_attB

   Plasmid

   #182145

   Purpose

   To insert attB attachment site at ALB in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   Alb_A277c_attB pegRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CDK2 gRNA (BRDN0001147786)

   Plasmid

   #77192

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK2 (CDK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRPPRC gRNA (BRDN0001147349)

   Plasmid

   #77774

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human LRPPRC

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   LRPPRC (LRPPRC Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-mCherry2-sgRNA (empty)

   Plasmid

   #198330

   Purpose

   Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   U6-sgRNA(F+E) empty
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2 EGFP sgRNA2

   Plasmid

   #164933

   Purpose

   Expresses EGFP sgRNA2 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. RPS6KA3 gRNA (BRDN0001144943)

   Plasmid

   #75942

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATM gRNA (BRDN0001149033)

   Plasmid

   #77531

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ATM

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ATM (ATM Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 EGFR-sgRNA

   Plasmid

   #188633

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RPS6KA3 gRNA (BRDN0001148452)

   Plasmid

   #75943

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATM gRNA (BRDN0001146099)

    Plasmid

    #77530

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYLsgRNA-OsU3m/LacZ

    Plasmid

    #66192

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    OsU3m (SpeI site destroyed)

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-P3-evoCjCas9-eAID_gRNA

    Plasmid

    #202562

    Purpose

    P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-eAID
    

    Use

    AAV

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT9658-sgRNA-mCherry

    Plasmid

    #162987

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MLKL gRNA (BRDN0001148608)

    Plasmid

    #77539

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. EIF2AK4 gRNA (BRDN0001146469)

    Plasmid

    #75876

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAPK1 gRNA (BRDN0001149093)

    Plasmid

    #77788

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MLKL gRNA (BRDN0001146456)

    Plasmid

    #77540

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. STK11 gRNA (BRDN0001147319)

    Plasmid

    #75914

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. eGFP L138 gRNA

    Plasmid

    #119133

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L138 gRNA
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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