We narrowed to 14,409 results for: eng
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Plasmid#197507PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
REKAR76
Plasmid#223262PurposeEKAREN4 modified to be a Red-FRET sensor using miRFP720 and miRFP670nano3 (in that order; AKA REKAR76)DepositorInsertREKAR-76
TagsmiRFP670nano3 and miRFP720ExpressionBacterial and MammalianMutationmiRFP720 - EKAREN4 - miRFP670nano3PromoterhEF-1aAvailable sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-roGFP
Plasmid#202464PurposeExpresses redox sensitive GFP (roGFP) with T7 inductionDepositorInsertreduction-oxidation sensitive green fluorescent protein
Tags6X-HisExpressionBacterialPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH613
Plasmid#169602PurposeTier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
ExpressionBacterialPromoterlacIqAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS416-Aga1p-FAPB2.3.6L1TAG
Plasmid#158148PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastMutationCloned in a TAG codon at the first position of th…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p158
Plasmid#62617PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Linear-GFP
Plasmid#226258PurposeProduction of linear RNA via IVTDepositorInsertEGFP
UseIn vitro transcriptionAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_D2A_tdTomato
Plasmid#184045PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsEnhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence
ExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIVT-iCre
Plasmid#232773PurposeIVT template for making modRNA encoding iCreDepositorInsertiCre
UseCre/Lox; In vitro transcriptionAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor
Plasmid#229787PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)DepositorInsertLacZ; ZF10-NS3-p65
UseSynthetic BiologyExpressionMammalianPromoterCAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1_IVT
Plasmid#222340PurposePlasmid for wild type Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized wtBxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)
Plasmid#197509PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSMutationABE8e mutations in TadA and nSpCas9(D10A)PromoterCbhAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM8314
Plasmid#213987PurposePrpsM driven expression of mRuby3DepositorInsertmRuby3
ExpressionBacterialPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only