We narrowed to 5,246 results for: golden gate

Showing: 1301 - 1320 of 5246 results

Results per page:

Sort results by:

L0-I42 Tp901 GG1-GG4

Plasmid

#195907

Purpose

Level 0 Tp901 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Tp901 integrae

Use

Synthetic Biology

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-I14 DST GG4-GG5

Plasmid

#195896

Purpose

Level 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

DST

Use

Synthetic Biology

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Publication

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6, CBh

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EC71102

Plasmid

#154065

Purpose

Level 0 Golden Gate vector; Unmodifed CRE

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE

Use

Synthetic Biology; Standard cloning vector used b…

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNAM-B1

Plasmid

#154056

Purpose

Level 0 Golden Gate vector, contains domesticated NAM-B1

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

TaNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Synonymous mutation at base 3257, in exon 3, to r…

Promoter

N/A

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131C-RZ-Lb

Plasmid

#134347

Purpose

Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

Depositor

Yiping Qi

Publication

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pFUSC8

Plasmid

#50428

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC7

Plasmid

#50427

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC6

Plasmid

#50426

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC5

Plasmid

#50425

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC4

Plasmid

#50424

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC3

Plasmid

#50423

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC2

Plasmid

#50422

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSC10

Plasmid

#50430

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132A2.0

Plasmid

#99887

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSB123 - pL0_rLUC-I (CDS1)

Plasmid

#123181

Purpose

Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants

Depositor

Erin Cram, Carolyn Lee-Parsons

Publication

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants

Use

Luciferase; Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites; codon-optimised for plants

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pDV052

Plasmid

#191825

Purpose

Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

Depositor

Jim Roberts

Publication

Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

Type

Empty backbone

Use

Unspecified

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

Plasmid

#181792

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133A2.0

Plasmid

#99891

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge