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pJB066

Plasmid

#98913

Purpose

Express FtsZ tagged with pAmCherry1 in E coli

Depositor

Jie Xiao

Publication

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

pAmCherry1

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1510

Plasmid

#91036

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

ZmUbi

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWT019a

Plasmid

#107893

Purpose

W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1

Expression

Bacterial

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-PT2A-GFP-iRFP670-Tdtomato

Plasmid

#111813

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
iRFP670
Tdtomato

Use

Retroviral

Expression

Mammalian

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pWT063a

Plasmid

#107908

Purpose

W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRESG-BE3

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR puroR R4-R3

Plasmid

#43965

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Available since

Sept. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR IRES L5-L4

Plasmid

#43964

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

IRES

Use

Multisite gateway entr clone

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR mCherry L1-R5

Plasmid

#43963

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

mCherry

Use

Multisite gateway entr clone

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMOD_C3001

Plasmid

#91094

Purpose

Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMOD_A0502

Plasmid

#91012

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pME-eSIBR

Plasmid

#71665

Purpose

intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

Depositor

Philip Washbourne

Publication

Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

Insert

intronic eSIBR cassette

Use

RNAi; Multi-site gateway

Promoter

None

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZA2[PLac:GFP]

Plasmid

#60778

Purpose

Contains Plac driving expression of GFP

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

yemGFP

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pTRANS_222

Plasmid

#91116

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-sgRNA(siteT9)

Plasmid

#154832

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

Depositor

Lise Marie Grav

Publication

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA(siteT9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R265

Plasmid

#138167

Purpose

Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(VDA).M86.(SDL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTK398

Plasmid

#114714

Purpose

mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLITMUS-rpoN-cI5G6T,P-J23106-geneIII

Plasmid

#80862

Purpose

Phagemid (encodes evolved cI5G6T,P and M13 gene III)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII

Plasmid

#80863

Purpose

Phagemid (encodes evolved cI4A5T6T,P and M13 gene III)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII

Plasmid

#80864

Purpose

Phagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5C6G7G,P

Expression

Bacterial

Mutation

238G to 238D (please see depositor comment below)

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Expression

Bacterial

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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