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We narrowed to 4,673 results for: pcr

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Showing: 1301 - 1320 of 4673 results

  1. pENTR221-miniCMVp-Blast-P2A-HaloTag

    Plasmid
    #251483
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation. MiniCMV promoter may enhance the expression of Halo-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    HaloTag7
    Use
    Synthetic Biology
    Promoter
    minimal CMV Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251483-plasmid-map-sequence-id-501113

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  2. pENTR221-sEFp-Blast-P2A-dTAG

    Plasmid
    #251484
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation. Short EF promoter enhances the expression of dTAG-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    dTAG
    Use
    Synthetic Biology
    Promoter
    short EF Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251484-plasmid-map-sequence-id-501127

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  4. S1PR2-Tango

    Plasmid
    #66497
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S1PR2 (S1PR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    66497-plasmid-map-sequence-id-344626

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  8. pcDNA3.1-Beta3

    Plasmid
    #140988
    Purpose
    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GBeta3 (GNB3 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    140988-plasmid-map-sequence-id-301591

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  9. GPR120-Tango

    Plasmid
    #66310
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR120 (FFAR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    66310-plasmid-map-sequence-id-248122

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  10. MRGPRX2-Tango

    Plasmid
    #66440
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRGPRX2 (MRGPRX2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles

  11. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

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  12. OPRK1-Tango

    Plasmid
    #66462
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPRK1 (OPRK1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66462-plasmid-map-sequence-id-157905

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  15. AGTR1-Tango

    Plasmid
    #66222
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AGTR1 (AGTR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    66222-plasmid-map-sequence-id-163593

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  16. pCMV-Sport6-CD81-pHluorin

    Plasmid
    #130903
    Purpose
    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD81-pHluorin (CD81 aequorea victoria, Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Mutation
    pHluorin inserted between Leu-49 and Gly-50 in ex…
    Promoter
    CMV
    Available since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    130903-plasmid-map-sequence-id-259050

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  17. pADH119

    Plasmid
    #90985
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90985-plasmid-map-sequence-id-180513

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  18. GPR110-Tango

    Plasmid
    #66302
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR110 (ADGRF1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66302-plasmid-map-sequence-id-314160

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  19. S1PR1-Tango

    Plasmid
    #66496
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S1PR1 (S1PR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66496-plasmid-map-sequence-id-347878

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  20. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Publication
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xCRE-CMVmin-luc2
    Expression
    Mammalian
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    194384-plasmid-map-sequence-id-381615

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Showing: 1301 - 1320 of 4673 results