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pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Promoter

Available since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-WT

Plasmid

#210722

Purpose

Construct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pMX GFP cdc42 Q61L

Plasmid

#14568

Depositor

Joan Brugge

Article

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

cdc42 Q61L (CDC42 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
GFP-pCdc42

Plasmid

#23240

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Cdc42 (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
GFP-bCdc42

Plasmid

#23241

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Cdc42 (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pRL K-ras 3'UTR mutant

Plasmid

#14805

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

K-ras 3'UTR (Kras Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

let-7 miRNA binding site mutations

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-Cdc42 3'UTR

Plasmid

#51389

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

Cdc42 3'UTR (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pUt-mNF-Cas13d

Plasmid

#224789

Purpose

LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

mCherry-P2A-RfxCas13d

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV-d2i

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-T17N

Plasmid

#214289

Purpose

Express dominant negative Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative Cdc42 (CDC42 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-T17N

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-Cdc42/N12

Plasmid

#214290

Purpose

Express split-Cdc42 fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-Cdc42 fragment fused with FRB (CDC42 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b-Cdc42/13C-FKBP-MBP

Plasmid

#214291

Purpose

Express split-Cdc42 fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-Cdc42 fragment fused with FKBP (CDC42 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-Q61L

Promoter

T7

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-W-ccdB-Z

Plasmid

#218893

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-YF

Plasmid

#210723

Purpose

Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

Plasmid

#182381

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX

Plasmid

#176121

Purpose

Constitutivley active, mitochondrial localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

CDC42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-EGFP-Cdc42-T17N

Plasmid

#12976

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 DN (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

CMV

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-EGFP-Cdc42-Q61L

Plasmid

#12986

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 constitutively active (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge