We narrowed to 2,433 results for: PRK

Showing: 1981 - 2010 of 2433 results

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pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4C

Plasmid

#67465

Purpose

Mammalian expression of hArl4c with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-parkin K161N

Plasmid

#227365

Purpose

For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin K161N (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin delta 101-109

Plasmid

#227364

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin delta 101-109 (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Resi…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

Plasmid

#212078

Purpose

LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8-exon (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mKO2-Parkin

Plasmid

#213544

Purpose

Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin(E16A)

Plasmid

#213546

Purpose

Transient mammalian expresssion of E16A Parkin

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

E16A mutation in Parkin

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

Plasmid

#184774

Purpose

Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3526

Plasmid

#145002

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

THAP12 (THAP12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα

Plasmid

#183454

Purpose

sgRNA targeting rat PKA-RIIα subunits

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA targeting rat PKA-RIIα (Prkar2a Rat)

Use

Lentiviral

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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HsGRK6[L66A E514A]-sYFP2

Plasmid

#137781

Purpose

Visualization of GRK6

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human GRK6[L66A E514A] (GRK6 Human)

Expression

Mammalian

Mutation

L66A E514A

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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myc-Miro2 (lacking TM)

Plasmid

#127614

Purpose

Human Miro2 with the transmembrane deleted

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro2 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

Transmembrane deletion

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11D)-mPAGFP

Plasmid

#114135

Purpose

Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11A)-mPAGFP

Plasmid

#114134

Purpose

Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN-mPAGFP

Plasmid

#114114

Purpose

Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSi-check2-hRac2 3'UTR

Plasmid

#97160

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

RAC 3'UTR (AKT1 Human)

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Tags

3xFLAG and NLS

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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MKNK2 gRNA (BRDN0001147215)

Plasmid

#77762

Purpose

3rd generation lentiviral gRNA plasmid targeting human MKNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MKNK2 (MKNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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MKNK2 gRNA (BRDN0001145258)

Plasmid

#77763

Purpose

3rd generation lentiviral gRNA plasmid targeting human MKNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MKNK2 (MKNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MKNK2 gRNA (BRDN0001148143)

Plasmid

#77764

Purpose

3rd generation lentiviral gRNA plasmid targeting human MKNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MKNK2 (MKNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MKNK2 gRNA (BRDN0001148155)

Plasmid

#77765

Purpose

3rd generation lentiviral gRNA plasmid targeting human MKNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MKNK2 (MKNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK3 gRNA (BRDN0001146119)

Plasmid

#76925

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK3 (WNK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK3 gRNA (BRDN0001148134)

Plasmid

#76926

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK3 (WNK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK3 gRNA (BRDN0001145371)

Plasmid

#76927

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK3 (WNK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK4 gRNA (BRDN0001147390)

Plasmid

#75657

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK4 (WNK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK4 gRNA (BRDN0001148891)

Plasmid

#75658

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK4 (WNK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK4 gRNA (BRDN0001148888)

Plasmid

#75659

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK4 (WNK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-ARL4

Plasmid

#67461

Purpose

Mammalian expression of hArl4 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Expression

Mammalian

Mutation

bp 105 A to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5A

Plasmid

#67462

Purpose

Mammalian expression of hArl5 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only