We narrowed to 2,550 results for: CCS

1. pHDR_FIGLA-T2A-mGreenLantern-PuroTK

   Plasmid

   #222905

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-cT3G-cERP2-SOX17

   Plasmid

   #222925

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-PRDM1

   Plasmid

   #222924

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   PRDM1 (PRDM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-ZGLP1

   Plasmid

   #222926

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZGLP1 (ZGLP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERH-SOHLH1

   Plasmid

   #222922

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   SOHLH1 (SOHLH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hygro-P2A-HA-FKBP-WAPL

   Plasmid

   #217666

   Purpose

   Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   WapI (WAPL Human)
   

   Use

   Tags

   dTag (FKBP36V protein)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Lac

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT_IFL_G1A-T2A_R

   Plasmid

   #200183

   Purpose

   WT PA14 I-F Leader and G1A-T2A Repeat

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G1A-T2A in the I-F repeat

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. WT_IFL_G1A-T2A-A28C_R

   Plasmid

   #200185

   Purpose

   WT PA14 I-F Leader and G1A-T2A-A28C Repeat

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G1A-T2A-A28C in the I-F repeat

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAW-457

   Plasmid

   #200213

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas1 H25A mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRAW-458

    Plasmid

    #200214

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 E184A mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAW-459

    Plasmid

    #200215

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 K214A mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAW-460

    Plasmid

    #200216

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 R55E-N56D mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRAW-461

    Plasmid

    #200217

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAW-462

    Plasmid

    #200218

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E, R55E-N56D mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-myc-EphB4

    Plasmid

    #200983

    Purpose

    Mammalian expression plasmid for myc-tagged EphB4

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB4 (EPHB4 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a(+)_co_L-HicDH

    Plasmid

    #185453

    Purpose

    CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

    Depositor

    Wolfgang Kroutil

    Article

    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

    Insert

    L-HicDH from Lactobacillus confusus DSM 20196
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid

    #185456

    Purpose

    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

    Depositor

    Wolfgang Kroutil

    Article

    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

    Insert

    D-HicDH from Lactobacillus paracasei DSM 20008
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc.x.tetO2

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sgPLXNB2

    Plasmid

    #86151

    Purpose

    Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304 PCSK5 (R511C)-V5 blast

    Plasmid

    #83104

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Arginine 511 to Cysteine

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only