We narrowed to 2,114 results for: D10

1. Stargazin-GFP-LOVpep

   Plasmid

   #80406

   Purpose

   Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

   Depositor

   Michael Glotzer

   Article

   Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

   Insert

   Stargazin-GFP-LOVpep (CACNG2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC154-dual-dCas9VP160-sgExpression

   Plasmid

   #48240

   Purpose

   Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A H840A

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

   Plasmid

   #211767

   Purpose

   dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pGK and pEF1a

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMEH31 Lamp2b HA

   Plasmid

   #168208

   Purpose

   Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT121_GalL_nCDA1-VQRBE3

   Plasmid

   #145086

   Purpose

   Expressing base editor nCDA1-VQRBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-VQRBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, D1135V, R1335Q, T1337R)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. YEE-BE4max

   Plasmid

   #138157

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YEE)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LAMP1-SEP-mRuby3

   Plasmid

   #200939

   Purpose

   endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   LAMP1 (LAMP1 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AALN-BE4max

   Plasmid

   #138161

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(AALN)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-OGT_opt

   Plasmid

   #190858

   Purpose

   For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells.

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Td-CGBE

    Plasmid

    #196599

    Purpose

    Mammalian expression of Td-CGBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L); S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ265E2

    Plasmid

    #164719

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTDH3-dCas9-Mxi1

    Plasmid

    #46921

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 myc card9

    Plasmid

    #16253

    Depositor

    William Kaelin

    Article

    Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

    Insert

    card9 (CARD9 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX302 OAS1-V5 puro

    Plasmid

    #158641

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS1 (OAS1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFl Strep-Sumo-TEV human OAS1

    Plasmid

    #130895

    Purpose

    Human oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobos

    Depositor

    Leemor Joshua-Tor

    Article

    Ando et al Mol Cell. 2019 Feb 21;73(4):845-856.e5.

    Insert

    oligoadenylate synthase 1 (OAS1 Human)
    

    Use

    Tags

    Strep_Sumo

    Expression

    Insect

    Mutation

    Promoter

    ph

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-hA3D-BE3

    Plasmid

    #113413

    Purpose

    Expresses hA3D-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3D-BE3 (APOBEC3D S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9 TadCBEe

    Plasmid

    #193836

    Purpose

    Express TadCBEe (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDe-SpCas9D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOPINB-OTULIN (full-length, aa 1-352)

    Plasmid

    #61464

    Purpose

    Expresses human OTULIN (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

    Insert

    OTULIN (OTULIN Human)
    

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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