We narrowed to 2,136 results for: PAM

Showing: 1321 - 1340 of 2136 results

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pAAV-hsyn-GRAB_rDA-mut

Plasmid

#140558

Purpose

Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-Ascl1-neomycin

Plasmid

#176482

Purpose

TetO-Ascl1 in a PiggyBac vector with neomycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Ascl1 (Ascl1 Mouse)

Expression

Mammalian

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pTH-iCre:EGFP-WPREpA

Plasmid

#213142

Purpose

Truncated rat TH promoter expressing iCre fused to EGFP

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-pTH-iCre-WPREpA

Plasmid

#213130

Purpose

Truncated rat TH promoter expressing iCre

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

Plasmid

#139987

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCWori cytochrome P450-BM3h 9D7

Plasmid

#61308

Purpose

Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

Depositor

Alan Jasanoff

Article

Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

Insert

Cytochrome P450-BM3 heme domain 9D7

Tags

6xHis

Expression

Bacterial

Mutation

T268A, I263A, T438V, A328G

Promoter

Tac

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

Plasmid

#184632

Purpose

Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad67 (Gad1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-Synaptojanin-1-145

Plasmid

#223563

Purpose

Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

SYNJ1 (SYNJ1 Human)

Tags

His and mCherry

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-R2505P

Plasmid

#69015

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-R2505P (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Arginine 2505 to Proline

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

Plasmid

#196677

Purpose

AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

PKIα-P2A-mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-L1460P

Plasmid

#69006

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-L1460P (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Leucine 1460 to Proline

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-I2500F

Plasmid

#69014

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-I2500F (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Isoleucine 2500 to Phenylalanine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight1.2

Plasmid

#111068

Purpose

To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mFoxa2

Plasmid

#33014

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Forkhead box A2 (Foxa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

H10L

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-GST-4EBP1-Low GC

Plasmid

#69018

Purpose

expresses tagged 4EBP1

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

EIF4EBP1 (EIF4EBP1 Human)

Tags

GST and HA

Expression

Mammalian

Mutation

lowGC content, wt is ~70% GC rich. Still, it code…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.1

Plasmid

#111067

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-MTOR-C1483F

Plasmid

#69008

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-C1483F (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Cysteine 1483 to Phenylalanine

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only