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pAc5.1B-lambdaN-HA-CeAIN2-W282A_O

Plasmid

#147141

Purpose

Insect Expression of CeAIN2-W282A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282A

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W282AW366A_O

Plasmid

#147142

Purpose

Insect Expression of CeAIN2-W282AW366A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282AW366A

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAV103-PRNR2-mCherry-Elk1DM-1SIM

Plasmid

#190252

Purpose

Expression mCherry-Elk1DM in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

ELK1DM-SIM

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV105-PPAB1-GFP-Erk1R84S-1SIM

Plasmid

#190253

Purpose

Expression Erk1-GFP-1SIM in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

ERK1R84S-SIM

Use

Tags

GFP

Expression

Yeast

Mutation

ERK1R84S

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2-W366AW390A_P

Plasmid

#147235

Purpose

Insect Expression of CeAIN2-W366AW390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W366AW390A

Use

Tags

Expression

Insect

Mutation

Deletion of two AA SD 378-379 compared to the seq…

Promoter

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-CeAIN1-W378AW437A_O

Plasmid

#147136

Purpose

Insect Expression of CeAIN1-W378AW437A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN1-W378AW437A (ain-1 Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN1_1-350_O

Plasmid

#147122

Purpose

Insect Expression of CeAIN1_1-350

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN1_1-350 (ain-1 Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-pduCDE

Plasmid

#110138

Purpose

Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

Insert

pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-tdTomato

Plasmid

#159275

Purpose

Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

Insert

tdTomato

Use

AAV; S1 knockin donor vector

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEY11

Plasmid

#191017

Purpose

Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

Ultrapanneuronal

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-sfGFP

Plasmid

#116917

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

J23101

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-Luc

Plasmid

#122694

Purpose

Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

Firefly luciferase

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

PBen

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

J23101

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST-HipO

Plasmid

#114599

Purpose

Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

HipO

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-CePABP_1-400_P

Plasmid

#147241

Purpose

Insect Expression of CePABP_1-400

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CePABP_1-400

Use

Tags

Expression

Insect

Mutation

one silent and one non silen mutation G392A compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmXRN1_1323-1355_P

Plasmid

#147245

Purpose

Bacterial Expression of DmXRN1_1323-1355

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

DmXRN1_1323-1355 (pcm Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1-1649_P

Plasmid

#147248

Purpose

Mammalian Expression of HsXRN1_1-1649

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

HsXRN1_1-1649 (XRN1 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ΔEMV-6MT

Plasmid

#41737

Depositor

Jeffrey Nye, Raphael Kopan

Article

Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

Insert

Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein fragment lacking …

Promoter

Available since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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