We narrowed to 1,518 results for: pet-28a

1. pJK593

   Plasmid

   #71587

   Purpose

   Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE )
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK618

   Plasmid

   #72535

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. C064

   Plasmid

   #174353

   Purpose

   Plasmid for expressing frog PABPC1 (C-His) in E.coli

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (pabpc1.S Frog)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NW11

   Plasmid

   #133443

   Purpose

   Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NW30

    Plasmid

    #133444

    Purpose

    Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW30
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpyCatcher003-mi3

    Plasmid

    #159995

    Purpose

    Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyCatcher003-mi3
    

    Use

    Tags

    C-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKS1

    Plasmid

    #84079

    Purpose

    Expresses the non-toxic fragment C of tetanus toxin

    Depositor

    Neil Fairweather

    Article

    Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

    Insert

    Tetanus neurotoxin fragment C
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Use

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MYST3

    Plasmid

    #25181

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MYST3_13 (KAT6A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains catalytic region ( residues 497-780).

    Promoter

    Available Since

    Oct. 25, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pDR119

    Plasmid

    #13332

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    DNA ligase IV (LIG4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

16. Human USP30 (64-502, construct 8, MG-26-21)

    Plasmid

    #110745

    Purpose

    Bacterial expression of human USP30 (construct 8)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350D, I353E + insertion deletion: 64-357;…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG247 His-iFAST

    Plasmid

    #130808

    Purpose

    Expresses His-tagged iFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

    Insert

    iFAST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tUI-HA

    Plasmid

    #122662

    Purpose

    Express tUI-HA protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI-HA
    

    Use

    Tags

    HA-tag and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Human RNF144A (16-228)

    Plasmid

    #110752

    Purpose

    Bacterial Expression of RNF144A RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144A (RNF144A Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpyTag003-mClover3

    Plasmid

    #133453

    Purpose

    Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mClover3
    

    Use

    Tags

    GSGSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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