We narrowed to 1,601 results for: tef

1. His SUMO TEV WT SPOP 28-359

   Plasmid

   #194115

   Purpose

   Wild type His-SUMO SPOP expression plasmid

   Depositor

   Tanja Mittag

   Article

   Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

   Insert

   SPOP (SPOP Human)
   

   Tags

   6His-SUMO-TEV

   Expression

   Bacterial

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MBII85-5'SS mut

   Plasmid

   #67631

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Expression

   Mammalian

   Mutation

   mutation in 5'ss

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHOHO2_Hsp82viiA

   Plasmid

   #61708

   Purpose

   Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiA (HSP82 Budding Yeast)
   

   Use

   Yeast integration vector targeting inserts to the…

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   TEF1

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXB1-EXO1b

   Plasmid

   #68267

   Purpose

   Bacterial expression of human EXO1

   Depositor

   Stefano Ferrari

   Article

   El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

   Insert

   EXO1 (EXO1 Human)
   

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   b form, codons 1-846. Please see depositor commen…

   Promoter

   T7

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

7. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. STAgR_Neo

   Plasmid

   #102992

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Promoter

   U6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM0646

    Plasmid

    #129007

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-FOXL2

    Plasmid

    #153135

    Purpose

    Expression of Flag tagged human FOXL2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXL2 (FOXL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPL5618_pUG_MtxR

    Plasmid

    #60929

    Purpose

    PCR template vector for generating methotrexate resistance cassette.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Dihydrofolate reductase (DHFR Human)
    

    Use

    Loxp flanked deletion cassette template

    Mutation

    L22F F31S

    Promoter

    TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. STAGR_SAMScaffold_hU6

    Plasmid

    #102843

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    As pcr template

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Promoter

    U6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV300

    Plasmid

    #204968

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-FOXE1

    Plasmid

    #153120

    Purpose

    Expression of Flag tagged human FOXE1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXE1 (FOXE1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21c(+)-EFG1mt

    Plasmid

    #31076

    Depositor

    Linda Spremulli

    Article

    Bhargava et al Protein Expr Purif. 2004 Oct . 37(2):368

    Insert

    Mature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes the coding sequence for the mature form.…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pMM0638

    Plasmid

    #129005

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-NES-mTagBFP2

    Plasmid

    #216129

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Promoter

    TDH3 and TEF1

    Available Since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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