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pCAG-HcRed

Plasmid

#11152

Purpose

Mammalian expression vector for expression of HcRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

HcRed1 from Heteractis crispa

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAP

Plasmid

#8340

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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LentiGuide Blast

Plasmid

#199622

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

Depositor

Danwei Huangfu

Article

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V1

Plasmid

#192474

Purpose

Lentiviral DSB Spectrum V1 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189925

Purpose

AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Use

AAV

Mutation

SauriCas9 D15A

Promoter

EFS

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon DREADD Gq-mCherry

Plasmid

#183532

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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spNW15

Plasmid

#173483

Purpose

Expresses spNW15 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW15

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Tags

GST

Expression

Bacterial

Promoter

lac promoter / tac promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHDM-Tat1b

Plasmid

#164442

Purpose

Encodes HIV-1 Tat accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Tat

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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