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We narrowed to 4,337 results for: ARA-2

Showing: 1341 - 1360 of 4337 results

  2. pVH004

    Plasmid
    #80401
    Purpose
    Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    CusR (cusR )
    AraC (araC )
    Anti-scaffold
    Use
    Synthetic Biology
    Tags
    C-terminal LZx domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80401-plasmid-map-sequence-id-155911

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  3. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  5. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

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  9. p5E-pth2

    Plasmid
    #191428
    Purpose
    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)
    Depositor
    Timothy Erickson
    Article
    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi
    Insert
    pth2 regulatory region (pth2 Zebrafish)
    Use
    Tol2 gateway 5' entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191428-plasmid-map-sequence-id-384465

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  10. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid
    #160139
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160139-plasmid-map-sequence-id-333510

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  11. pVH016

    Plasmid
    #80400
    Purpose
    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal 3xFLAG tag and C-terminal LZx domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    80400-plasmid-map-sequence-id-155905

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  12. attB-P7-attP-YFP

    Plasmid
    #195571
    Purpose
    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.
    Depositor
    Richard Murray
    Article
    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52
    Insert
    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    P7
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    195571-plasmid-map-sequence-id-383991

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  13. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid
    #160138
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160138-plasmid-map-sequence-id-333508

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  17. pVH015

    Plasmid
    #80398
    Purpose
    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal 3xFLAG tag and C-terminal LZx domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    80398-plasmid-map-sequence-id-155896

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  18. pDGB1alpha11_SulR

    Plasmid
    #186424
    Purpose
    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Insert
    SulR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186424-plasmid-map-sequence-id-369067

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  20. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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Showing: 1341 - 1360 of 4337 results