We narrowed to 2,684 results for: Kl

1. mTagRFP-T-Actin-7

   Plasmid

   #58001

   Purpose

   Localization: Actin, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pH7WG2B-OsMIR390-B/c

   Plasmid

   #61465

   Purpose

   Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Use

   Tags

   Expression

   Plant

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Promoter

   OsUBI

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nSaCas9 I744 miniABEMax V82G

   Plasmid

   #135379

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 I744 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mOrange2-Golgi-7

   Plasmid

   #57959

   Purpose

   Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

   Plasmid

   #136513

   Purpose

   Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

   Insert

   codon-optimized AcrIIA5
   

   Use

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME DN-E-cadherin

   Plasmid

   #135209

   Purpose

   Dominate Negative zebrafish e-cadherin label

   Depositor

   David Tobin

   Article

   Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

   Insert

   CDH1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. E12-cTAP

   Plasmid

   #20916

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   E12 cTAP (Tcf3 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TAP tag is fused to the 3' end of the E12 ge…

   Promoter

   Available since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pBAD mApple

   Plasmid

   #91772

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   monomeric Apple
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   R97K, E152S, A180S, V207T compared to mApple (FP …

   Promoter

   araBAD

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDC32B-OsMIR390-B/c

    Plasmid

    #61467

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    2x35S

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEJS1026-pMCSG7-HpaCas9

    Plasmid

    #121540

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    HpaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA1-Bystin

    Plasmid

    #11869

    Depositor

    Michiko Fukuda

    Article

    Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

    Insert

    Bystin (BYSL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

13. mOrange2-Fibrillarin-7

    Plasmid

    #57957

    Purpose

    Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAcrF2

    Plasmid

    #89234

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    AcrF2
    

    Use

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMDC123SB-OsMIR390-B/c

    Plasmid

    #61466

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    2x35S

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mOrange2-LaminB1-10

    Plasmid

    #57966

    Purpose

    Localization: Nuclear Envelope, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. mOrange2-Keratin-17

    Plasmid

    #57965

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP TEM4 R130D

    Plasmid

    #58895

    Purpose

    Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.

    Depositor

    Channing Der

    Article

    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

    Insert

    TEM4 (ARHGEF17 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R130D, L722F

    Promoter

    CMV

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTagRFP-T-Fibrillarin-7

    Plasmid

    #58016

    Purpose

    Localization: Nucleoli, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits