We narrowed to 3,176 results for: UBC

1. pHHYTK128

   Plasmid

   #200140

   Purpose

   Type 4a part for Modular Cloning in Yeast

   Depositor

   Johannes Herrmann

   Article

   Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

   Insert

   Ubc6-TA (233-250)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. YCe1897 HC_Kan_C-KDEL_8a

   Plasmid

   #100681

   Purpose

   part designed to occupy position 8a of EMMA. Functional category: Signal peptide for subcellular targeting

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   C-KDEL
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. YCe1903 HC_Kan_SV40-NLS_p6

   Plasmid

   #100684

   Purpose

   part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   SV40-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. YCe2058 HC_Kan_Kozak_ATG_Palm_p6

   Plasmid

   #100694

   Purpose

   part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   Kozak_ATG_Palm
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFOSGE_COF1G03

   Plasmid

   #70794

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   vih (vih Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBI121-GFP-RmMYB108

   Plasmid

   #173181

   Purpose

   Expresses Rosa multiflora MYB108 for subcellular localization in plants

   Depositor

   Daidi Che

   Article

   Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

   Insert

   RmMYB108
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Reza Zolfaghari, Catharine Ross

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HPV-31neo

   Plasmid

   #153283

   Purpose

   Neomycin selectable HPV31 genome

   Depositor

   Elliot Androphy

   Article

   Gilson et al J Virol. 2020 Jul 30;94(16). pii: JVI.00

   Insert

   HPV-31 genome with Neo cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   HindIII and CsiI site mutated from original Neo c…

   Promoter

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV GG hUbV-EGFP

    Plasmid

    #216161

    Purpose

    Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG-HHARI(1-185)

    Plasmid

    #17341

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-185

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pHH0103_UBA52_Ubiquitin

    Plasmid

    #110043

    Purpose

    Protein expression and purification of UBA52_Ubiquitin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    UBA52_Ubiquitin (UBA52 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSC-hTDG-K330A-PreE2

    Plasmid

    #52273

    Purpose

    Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Use

    Tags

    Ubc9-GST

    Expression

    Bacterial

    Mutation

    K330A, SUMO acceptor site mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSC-hTDG-IntE2

    Plasmid

    #52270

    Purpose

    Bacterial expression of human TDG C-terminally fused to GyrA intein, SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Use

    Tags

    GyrA-intein-Ubc9-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. empty_pK7fWG2_IVD_FP611

    Plasmid

    #228371

    Purpose

    Empty dual expression vector for determining subcellular localization for protein of interest

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Type

    Empty backbone

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. EKAREN5-gl

    Plasmid

    #225958

    Purpose

    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

    Depositor

    Toru Hiratsuka

    Article

    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

    Insert

    EKAREN5-gl
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherryC1-Pacer

    Plasmid

    #216698

    Purpose

    Mammalian expression of Pacer

    Depositor

    James Hurley

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    Pacer (RUBCNL Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGG-TurboID-GFP

    Plasmid

    #209399

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5’UTR-ORF1

    Plasmid

    #190566

    Purpose

    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    

    Use

    For dna propagation only

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. Negative feedback (NF) plasmid

    Plasmid

    #169747

    Purpose

    Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSB1C3-ysg(NT)

    Plasmid

    #138263

    Purpose

    Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YCe1885 HC_Kan_Kozak-ATG-MLS_p6

    Plasmid

    #100676

    Purpose

    part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    Kozak-ATG-MLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YCe2735 HC_Kan_Kozak-ATG-IgkL_p6

    Plasmid

    #100697

    Purpose

    part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    Kozak_ATG_IgkL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFOSGE_COF2B08

    Plasmid

    #70910

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Uba2 (Uba2 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pSC-hXRCC1-K176R-PreE2

    Plasmid

    #52279

    Purpose

    Bacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    Ubc9-GST

    Expression

    Bacterial

    Mutation

    K176R, SUMO acceptor site mutant

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC-hXRCC1-IntE2

    Plasmid

    #52276

    Purpose

    Bacterial expression of human XRCC1 C-terminally fused to GyrA intein, SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GyrA-intein-Ubc9-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCold-TF-hRubicon

    Plasmid

    #64147

    Purpose

    For protein expression in E. coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid

    #78250

    Purpose

    Can be used to subclone in gene of interest into blue motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    

    Use

    Zebrafish motor neuron expression

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196988

    Purpose

    Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    Nestin-dCas9-KRAB-T2a-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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