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Plasmid#109582PurposeMultisite gateway vector for 3' tagging with dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
RP125 pUAST EGFP:Orco
Plasmid#53441PurposeN-terminal fusion of EGFP to Drosophila melanogaster OrcoDepositorInsertEGFP:Orco (Orco Fly)
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-AtC60αβC20NtL
Plasmid#160876PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-FAPP1
Plasmid#109503PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertactc1b-mKate2-FAPP1
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-RhoAb-DN
Plasmid#109580PurposeMultisite gateway vector for 3' tagging with dominant negative RhoAbDepositorInsertRhoAb (rhoab Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LC001: pMVP/PB/Hygro-DEST
Plasmid#121876PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST EIF2C3
Plasmid#19889DepositorInsertEukaryotic translation initiation factor 2C, 3 (AGO3 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT7
Plasmid#53111PurposeExpression of Drosophila 5-HT7DepositorInsert5HT7 (5-HT7 Fly)
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMP416
Plasmid#134996PurposedCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)DepositorInsertpCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
UseSynthetic BiologyTags3xFLAG, E coli Dam(N132A), and dCas9ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-2xFYVEhgs
Plasmid#109505PurposeMuscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3PDepositorInsertactc1b-mKate2-2xFYVEhgs
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT1B
Plasmid#53108PurposeExpression of Drosophila 5-HT1BDepositorInsert5HT1B (5-HT1B Fly)
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT2A
Plasmid#53109PurposeExpression of Drosophila 5-HT2ADepositorInsert5HT2A (5-HT2 Fly)
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT1A
Plasmid#53107PurposeExpression of Drosophila 5-HT1ADepositorInsert5HT1A (5-HT1A Fly)
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ING2
Plasmid#109554PurposeMultisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsert3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab32a
Plasmid#109621PurposeMuscle specific zebrafish expression of mKate2 fused to rab32aDepositorInsertrab32a (rab32a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab14
Plasmid#109600PurposeMuscle specific zebrafish expression of mKate2 fused to rab14DepositorInsertrab14 (rab14 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM9-MAPKK2-WT
Plasmid#40805DepositorInsertMAP2K2 (MAP2K2 Human)
UseTagsHAExpressionMammalianMutationWild typePromoterMSV-LTRAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK
Plasmid#154454PurposeExpression of renilla luciferase and firefly luciferaseDepositorInsertRenilla luciferase, firefly luciferase
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_TFE3_2
Plasmid#86326PurposeEncodes gRNA for 3' target of human TFE3DepositorInsertgRNA against TFE3 (TFE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2B2
Plasmid#160880PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXNtTcR1AtR2NtB2
Plasmid#160889PurposeExpression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-Tc
UseTagsExpressionBacterialMutationPromoterrbcS-Tc: T7, chaperones: T12Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
JL202: pMVP/mCherry-DEST
Plasmid#121853PurposepMVP destination vector, empty expression vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JL002: pMVP/eGFP-DEST
Plasmid#121852PurposepMVP destination vector, empty expression vector backbone w/ eGFP selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-lamp1-mKate2
Plasmid#109507PurposeMuscle specific zebrafish expression of mKate2 fused to lamp1DepositorInsertlamp1 (lamp1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterActin5CAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
KN206: pMVP/eCFP-DEST
Plasmid#121854PurposepMVP destination vector, empty expression vector backbone w/ eCFP selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JC001: pMVP/Ad/RGD-DEST
Plasmid#121844PurposepMVP destination vector, empty RGD fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC267
Plasmid#118887Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tagDepositorInsertNcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; HALO tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-26 (GB1001)
Plasmid#68208PurposeProvides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-26 promoter
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT2R1AtR2NtB2
Plasmid#160886PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T2
UseTagsExpressionBacterialMutationPromoterrbcS-T2: T7, chaperones: T9Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD
Plasmid#73300PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationN1043S-Q1047E-S1079N-E1083QPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
JH401: pMVP/Ad/RGD-PK-DEST
Plasmid#121846PurposepMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCL-HA-MAPKK2-KW71[delta(48-55)/S222D/S226D]
Plasmid#40821DepositorInsertMAP2K2 (MAP2K2 Human)
UseTagsHAExpressionMammalianMutationdelta(48-55) + S222D + S226DPromoterCMVAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only