We narrowed to 14,584 results for: pET

1. pET28b+3(R112C/T180C)C

   Plasmid

   #108193

   Purpose

   Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b+C3(R112C/T180C)X

   Plasmid

   #108196

   Purpose

   Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   P450cam-PCNA3(R112C/T180C)-PdX fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3); C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-Metavinculin tail

   Plasmid

   #107152

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

   Insert

   metavinculin (VCL Human)
   

   Use

   Tags

   (His)8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-EPN01(∆M)-His

   Plasmid

   #84510

   Purpose

   E.coli version of EPN01(∆M)

   Depositor

   Neil King

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN01(∆M)-His
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET15b-E228A-PvuRts1I

   Plasmid

   #86896

   Purpose

   express E228A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E228A

   Promoter

   T7

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET15b-W215A-PvuRts1I

   Plasmid

   #86898

   Purpose

   express W215A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W215A

   Promoter

   T7

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET15b-E71A-PvuRts1I

   Plasmid

   #86895

   Purpose

   express E71A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E71A

   Promoter

   T7

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b-D57A-PvuRts1I

   Plasmid

   #86892

   Purpose

   express D57A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   D57A

   Promoter

   T7

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15b-R208A-PvuRts1I

   Plasmid

   #86891

   Purpose

   express R208A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   R208A

   Promoter

   T7

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b-E20A-PvuRts1I

    Plasmid

    #86897

    Purpose

    express E20A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    Pvurts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E20A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b-D70A-PvuRts1I

    Plasmid

    #86890

    Purpose

    express D70A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D70A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. csd2_121-308/pET21a(+)

    Plasmid

    #83293

    Purpose

    csd2, construct: 121-308, pET21a(+) no-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. csd5_41-192/pET28b(+)

    Plasmid

    #83296

    Purpose

    csd5, construct: 41-192, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    hp1250
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-40

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. csd4_22-438/pET28b(+)

    Plasmid

    #83295

    Purpose

    csd4, construct: 22-438, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    hp1075
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-21

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

15. csd2_140-251/pET21a(+)

    Plasmid

    #83294

    Purpose

    csd2, construct:140-251, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-139 and 252-308

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. csd1_54-312/pET21a(+)

    Plasmid

    #83291

    Purpose

    csd1, construct: 54-312, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-53

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. csd2_121-308/pET28b(+)

    Plasmid

    #83292

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. csd1_125-312/pET28b(+)

    Plasmid

    #83290

    Purpose

    csd1, construct: 125-312, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-124

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDuet-msGC-NT2

    Plasmid

    #74763

    Purpose

    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

    Depositor

    William Montfort

    Article

    Hu et al J Biol Chem. 2008 Jul 25;283(30):20968-7

    Inserts

    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    fragment 49-471 of the alfa subunit

    Promoter

    T7

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK044 (pET-PurQ-wt)

    Plasmid

    #73839

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETM6-3H5-mCherry

    Plasmid

    #73419

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3H5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3H5 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET23a-MmUBB(SnaBI)

    Plasmid

    #69558

    Purpose

    In vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The two last codons (TATTAA; encoding the last ty…

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pET23a-MmUBB(Y305P)

    Plasmid

    #69557

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon TAT encoding tyrosine 305 was changed to CC…

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pET151-hUCH37 (ISF2)

    Plasmid

    #61930

    Purpose

    Expression of hUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pET30Xa-SBL1(160E)

    Plasmid

    #66846

    Purpose

    Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28-p53 (Cow 1-82)

    Plasmid

    #62062

    Purpose

    Expression of Cow p53 (residues 1-82) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-82

    Promoter

    T7

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pET28-p53 (Rabbit 1-87)

    Plasmid

    #62058

    Purpose

    Expression of Rabbit p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pET28A-p53 (1-73) (PPP)

    Plasmid

    #62067

    Purpose

    Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A, P27A

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pET28-ceRab2(1-214)

    Plasmid

    #59550

    Purpose

    makes 6x Fusion of Full length ceRab2

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-214
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pET28-ceRab5(1-208)

    Plasmid

    #59551

    Purpose

    makes 6x Fusion of Full length ceRab5

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-208
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pET28-ceRab10(1-201)

    Plasmid

    #59552

    Purpose

    makes 6x Fusion of Full length ceRab10

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-208
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only