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pet 3d beta tentacle delete CP

Plasmid

#13300

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)

Expression

Bacterial

Mutation

CP alpha subunit is intact with a mutated beta C-…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

Plasmid

#233418

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233435

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233426

Purpose

PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Plasmid

#233424

Purpose

IVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ034 CMV-TO-CCL20

Plasmid

#233416

Purpose

plasmid expressing secreted soluble CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20 (CCL20 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

Plasmid

#233381

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR2(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

Plasmid

#233382

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR1(ecto+TM)-ADAR2dd

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

Plasmid

#233386

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rb(ecto+TM)-ADAR2dd

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

Plasmid

#233389

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rg(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Jarid2-polyA

Plasmid

#223811

Purpose

IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

Depositor

Azusa Inoue

Article

Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

Insert

Jarid2 (Jarid2 Mouse)

Use

In vitro transcription

Tags

Flag

Expression

Mammalian

Promoter

CMV, T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Ubc-c005

Plasmid

#216876

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c004

Plasmid

#216875

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

Plasmid

#208111

Purpose

Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

SpG C, U6, scaffold

Use

AAV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN

Plasmid

#206967

Purpose

Expresses TadA7.10 and SpG cas9N by the constitutive CMV promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

CMV, TadA7.10, SpG N

Use

AAV

Promoter

CMV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TadA8e-SpG N aa2-713-InteinN

Plasmid

#206968

Purpose

Expresses TadA8e and SpG cas9N by the constitutive CMV promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

CMV, TadA8e, SpG N

Use

AAV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-Lmna sgRNA

Plasmid

#206979

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

Lmna sgRNA

Use

AAV; Adenine base editor

Promoter

U6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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REEP6-2Strep

Plasmid

#210351

Purpose

Expresses REEP6 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP6 (REEP6 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)

Plasmid

#180029

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Halo-H2DBD

Plasmid

#200206

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-H2DBD (Hsf2 Mouse)

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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