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Plasmid#228120PurposeExpress node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK62
Plasmid#228122PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK55
Plasmid#228114PurposeExpress node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV272
Plasmid#226183PurposeExpresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225645PurposetetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA and LacO3O1 promotersAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225648PurposeLacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1 and tetR/tetA in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromoterLacO3O1 and tetA promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225647PurposeLacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1 and tetR/tetA in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromoterLacO3O1 and tetA promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225646PurposetetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA and LacO3O1 promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
UseTagsExpressionBacterialMutationS226P/R228SPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
RoL10X_pET29b
Plasmid#215427PurposeExpression construct for RoL10X gene, codon optimized for expression in E. coliDepositorInsertRoL10X
UseTagsExpressionBacterialMutationV209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB-SCY_pET29b
Plasmid#215415PurposeExpression construct for NylB-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
UseTagsHis tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroMD-mRuby2-ST
Plasmid#171158PurposeCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroMD
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-E222A-E272A
Plasmid#35060DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; E222A and E272A…PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-Y218A-Y267A
Plasmid#35059DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; Y218A and Y267A…PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-E222A-E272A-E381A
Plasmid#35061DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; E222A, E272A, a…PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG72QDProCFPp303
Plasmid#15590DepositorInserthtt 72Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176628PurposeTF A self-activation cassette in MultiFate-2.3DepositorInsert14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176624PurposeTF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3DepositorInsertTRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
Plasmid#176629PurposeTF C self-activation cassette in MultiFate-3DepositorInsert14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only