We narrowed to 4,114 results for: SPL

Showing: 1341 - 1360 of 4114 results

Results per page:

Sort results by:

pTRE-Tight-FLAG-CYLD-C601A

Plasmid

#60029

Purpose

CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

FLAG

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

TRE

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140230

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg (reverse orientation)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GST PTBP1 isoform 4

Plasmid

#108594

Purpose

Recombinant GST tagged protein production

Depositor

Chris Smith

Article

Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

Insert

PTBP1 isoform 4 (PTBP1 Human)

Tags

GST

Expression

Bacterial

Mutation

isoform 4 (isoform a)

Promoter

tac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV iGluf

Plasmid

#105833

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXIN-Ap1g1-FKBP

Plasmid

#46946

Purpose

Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap1g1-FKBP

Use

Retroviral

Tags

Mainly mouse Ap1g1 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SIN-BX SpyCatcher003-(short)mCD80

Plasmid

#210567

Purpose

Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TRIPZ-CTID195-GSQ-SUMO1nc-PURO

Plasmid

#208035

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcI-ts,ind+-(mtSSB delta20)

Plasmid

#186280

Purpose

Expresses Human Mitochondrial SSB in E. coli

Depositor

Kenneth Johnson

Article

Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

Insert

Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)

Expression

Bacterial

Mutation

First 20 amino acids corresponding to probable mi…

Promoter

lambda

Available Since

July 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Venus-tBid-pEGFP-C1

Plasmid

#166736

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

tBid (Bid Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CaMKIV-DCTK75E

Plasmid

#126423

Purpose

A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

Depositor

Douglas Black

Article

Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

Insert

calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed lysine 75 to glutamic acid

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC-I2Cas9

Plasmid

#173158

Purpose

Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Inserts

hSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region

Tags

3xFLAG-NLS and NLS

Expression

Plant

Mutation

Contains a D169N mutation with no visible effect …

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTomatoSlicer

Plasmid

#221085

Purpose

Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMito.iGABASnFR.F102G.mRuby3

Plasmid

#112180

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Tags

mRuby3

Expression

Mammalian

Mutation

F102G

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

Plasmid

#205445

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-Crry-mCD59-Qa1 SCT

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-68_HNF4A2

Plasmid

#31092

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_CO-Mini-C

Plasmid

#190337

Purpose

Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-C, PCSK9 +1A insertion pegRNA

Use

AAV

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZMU-Dest

Plasmid

#105089

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2014;1062:659-78. doi:

Type

Empty backbone

Tags

myc

Expression

Yeast

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSM101

Plasmid

#48791

Purpose

NHEJ reporter plasmid for 5' and 3' cohesive ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEC779

Plasmid

#234348

Purpose

Modular RENDR, GFP output, targets synthetic target

Depositor

James Chappell

Article

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Modular RENDR, GFP output

Use

Synthetic Biology

Promoter

PJ23119

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEC780

Plasmid

#234349

Purpose

Expressed synthetic RNA target for RENDR

Depositor

James Chappell

Article

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Synthetic RNA target for RENDR

Use

Synthetic Biology

Promoter

PJ23119

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge