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pCellFree_G03 MAPK13

Plasmid

#67077

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

MAPK13 (MAPK13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLTR PKC epsilon

Plasmid

#8420

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 ATF3

Plasmid

#67057

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ATF3 (ATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

M2I

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CAMK1

Plasmid

#67075

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CAMK1 (CAMK1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMTH PKC gamma

Plasmid

#8412

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pLTR PKC gamma

Plasmid

#8418

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-Gluc-feRMA-IRES-EGFP

Plasmid

#248190

Purpose

Expresses Gluc-feRMA and EGFP under the hSyn promoter. Gluc-feRMA enables monitoring of neuronal transduction and includes a TEV cs, allowing inactivation of it upon interaction with TEV protease.

Depositor

Jerzy Szablowski

Article

Nouraein et al bioRxiv 2025.05.08.652140

Inserts

Gaussia luciferase with TEV cleavage site fused to IgG1 Fc
IRES-EGFP

Use

AAV; Luciferase

Tags

Gluc (N terminal on insert) and IgG1 Fc (C termin…

Expression

Mammalian

Promoter

IRES and hSyn

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pT7RNAPNev-NB1

Plasmid

#216336

Purpose

Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

Depositor

Mark Styczynski

Article

McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

Insert

T7RNAPN-NB1 fusion

Expression

Bacterial

Available Since

July 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-Cluc-NHP.RMA

Plasmid

#227888

Purpose

Expresses Cluc-NHP.RMA under the hSyn promoter. Cluc-NHP.RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/

Insert

Cypridina luciferase fused to macaque Fc

Use

AAV and Luciferase

Tags

Cluc, IgG1 Fc, and T7 tag

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-A-V5-His

Plasmid

#222849

Purpose

E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-A

Tags

V5-His

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p931-SWITCH-OVER insert: EF1a-Blasti

Plasmid

#217890

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p223-Pulse-Switch

Plasmid

#217889

Purpose

Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA

Plasmid

#183802

Purpose

AAV vector to express nuclear localized dsRed-Express from CaMKIIa promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

dsRed-Express

Use

AAV

Tags

NLS

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-2X(CMV-eBFP2-3XNLS-pA)

Plasmid

#195867

Purpose

to express nuclear localized eBFP2

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

eBFP2

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-IC2N-dCer3

Plasmid

#191325

Purpose

Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

IC2N-dCer-FRB (Dync1i2 Mouse)

Expression

Mammalian

Mutation

aa 1-237

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-BAF

Plasmid

#164248

Purpose

retroviral plasmid for GFP-BAF

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

BAF (BANF1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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