We narrowed to 6,501 results for: nls

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pNXRVb-HACre

Plasmid

#78401

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
hygMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET-deSpCas9-VP64-6xHis

Plasmid

#92117

Purpose

Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

T7

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

Plasmid

#205281

Purpose

CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.

Depositor

Matthias Prigge

Insert

GCaMP6s

Use

AAV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2497

Plasmid

#190378

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::QacR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Publication

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::QacR-NLS_19St

Expression

Plant

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK047

Plasmid

#171319

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed-NLS fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK057

Plasmid

#171329

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4)-VPR-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK044

Plasmid

#171316

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP-NLS fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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S3_002_026_096

Plasmid

#134403

Purpose

pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

None and none

Expression

Yeast

Mutation

None and none

Promoter

pGPM1 and pRPL18B

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBALU18

Plasmid

#69334

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOP

Depositor

Oliver Hobert

Publication

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

rSL2-NLS-mChOint-FgF

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pSLQ12701-PHR-EF1a-CD19-GS-EGFP-P2A-puroR-T2A-BFP-NLS

Plasmid

#252637

Purpose

Human expression vector containing EF1a promoter, CD19 that is fused to GFP, nuclear BFP and puromycin resistance gene

Depositor

Stanley Qi

Publication

Chen et al Nat Cell Biol. 2026 May;28(5):1076-1086.

Insert

CD19-EGFP, PuroR, BFP-NLS

Use

Lentiviral

Promoter

EF1a

Available since

April 28, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

Plasmid

#203541

Purpose

gRNA expression in astrocytes

Depositor

Ben Deverman

Publication

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

NLS-GFP, 2x U6-RNA

Use

AAV

Expression

Mammalian

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E6-dTom-nlsdTom

Plasmid

#135641

Purpose

AAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has service

AAV1

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1, 5F-L YFP

Plasmid

#84913

Purpose

Mammalian expresion of TDP-43 NLS1, 5F-L YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Publication

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR4-HA-myc-nuc (w557-1)

Plasmid

#20025

Purpose

Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

HA, Myc, and NLS

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Publication

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway Cloning

Available since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_mCherry2-NLS-PEST_Puro

Plasmid

#213773

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Publication

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_mCherry2-NLS-PEST_Puro

Plasmid

#213769

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Publication

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_mCherry2-NLS-PEST_Puro

Plasmid

#213767

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Publication

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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