We narrowed to 9,223 results for: yeast expression

1. pYZ462

   Plasmid

   #187970

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-TID-HA3-HIS3MX6

   Plasmid

   #227981

   Purpose

   To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pG4

   Plasmid

   #162605

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pG2

   Plasmid

   #162603

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-TID-HA3-KanMX6

   Plasmid

   #227980

   Purpose

   To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YIplac211-yoHaloTag-TLG1

   Plasmid

   #105277

   Purpose

   Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Tlg1 (TLG1 Budding Yeast)
   

   Use

   Tags

   yoHaloTag

   Expression

   Yeast

   Mutation

   HaloTag has yeast optimized codons

   Promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDRF1-GW yAT1.03

   Plasmid

   #132781

   Purpose

   FRET biosensor for ATP measurements in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

   Insert

   yAT1.03 ymTq2-tdTomato
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-link-yoPA-mCherry-Kan

   Plasmid

   #44950

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPA-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-link-yoTagRFP-T-CaURA3

   Plasmid

   #44877

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoTagRFP-T
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWL89A mPing

    Plasmid

    #145787

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Susan Wessler, Charles Nathan Hancock

    Article

    Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

    Insert

    mPing
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pIM23

    Plasmid

    #74643

    Purpose

    Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BS-Ura3Kl

    Plasmid

    #69195

    Purpose

    complementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Kluyveromyces lactis URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-mTID-HA3-HIS3MX6

    Plasmid

    #227983

    Purpose

    To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    miniTurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV0584

    Plasmid

    #133471

    Purpose

    Integration into fission yeast genome at ura4 locus with natMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-link-yoTagRFP-T-SpHis5

    Plasmid

    #44842

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-mTID-HA3-KanMX6

    Plasmid

    #227982

    Purpose

    To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    miniTurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-link-yoLSS-mKate2-Kan

    Plasmid

    #44943

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAEF5

    Plasmid

    #136305

    Purpose

    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge