We narrowed to 8,596 results for: Set

1. pDualEX-SMN2

   Plasmid

   #214431

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM65879

   Plasmid

   #153214

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDualIN-SMN2

   Plasmid

   #214430

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM65881

   Plasmid

   #153215

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCS mOrange

   Plasmid

   #91764

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Orange
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-AAVS1-Ef1a-Puro

   Plasmid

   #227266

   Purpose

   Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT7

   Plasmid

   #204292

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGE_En03

   Plasmid

   #71535

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Sumihare Noji

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-tdTomato

   Plasmid

   #159275

   Purpose

   Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

   Insert

   tdTomato
   

   Use

   AAV; S1 knockin donor vector

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSNA

    Plasmid

    #135192

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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