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pLentiCMV Puro DEST ERKKTRClover

Plasmid

#59150

Purpose

Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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JD639

Plasmid

#160398

Purpose

Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis miR396-resistant GRF4-GIF1 chimera

Use

Binary vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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tetO-TBX5

Plasmid

#46032

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

TBX5 (TBX5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-hTERT-HA

Plasmid

#1772

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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aCKAR

Plasmid

#124427

Purpose

FRET-based reporter for monitoring aPKC activity in cells

Depositor

Alexandra Newton

Article

Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

Insert

aCKAR

Use

Tags

mCFP and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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JD631

Plasmid

#160399

Purpose

Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis GRF4-GIF1 chimera

Use

Binary vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-ExRai-AKAR

Plasmid

#118405

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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BRCA1 (1-304)

Plasmid

#12645

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

E3-Ring domain, Ub-ligase (BRCA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Use

Tags

Citrine and ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR

Plasmid

#118470

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFB-LIC-Bse

Plasmid

#26108

Purpose

SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Type

Empty backbone

Use

Baculovirus expression

Tags

His6 - TEV

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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tetO-Myocd

Plasmid

#46033

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Myocd (Myocd Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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CrBlue ---- pcDNA3.1

Plasmid

#62917

Purpose

CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNAintron-EBOV-GP

Plasmid

#215692

Purpose

Produces the Ebola virus glycoprotein

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

Insert

EBOV-GP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)-sapphireAKAR

Plasmid

#118464

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE2-Hyg-omMx1

Plasmid

#52420

Purpose

Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

Depositor

Bertrand Collet

Article

Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

Insert

Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE2

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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ROZA YF

Plasmid

#64197

Purpose

Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA YF

Use

Tags

Expression

Mammalian

Mutation

tyrosine phosphorylatin site mutated to phenylala…

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pBABE-neo-hTERT

Plasmid

#1774

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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