We narrowed to 5,173 results for: pcDNA3.1+

1. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

   Plasmid

   #168189

   Purpose

   HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

   Plasmid

   #49065

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

   Plasmid

   #193999

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181-D174N

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181-D174N
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

   Plasmid

   #166801

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   S730A

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MUC2 D1D2D' with cleavable His tag

   Plasmid

   #187993

   Purpose

   Expresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cells

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Mucin2 D1D2D' (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

   Plasmid

   #69903

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

   Plasmid

   #126444

   Purpose

   Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

   Depositor

   Jeremy Wilusz

   Article

   Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

   Insert

   Fndc3b (Fndc3b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   circFndc3b exons are separated by a miniature int…

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

   Plasmid

   #49063

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. XE252 pCDNA3.1(zeo)-hDsh3-333-716

   Plasmid

   #16757

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-333-716-Dsh3 (DVL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 333-716

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X

    Plasmid

    #16759

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    VSV-KLHL12-Q405X (KLHL12 Human)
    

    Use

    Tags

    vsv

    Expression

    Mammalian

    Mutation

    Q405STOP

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG

    Plasmid

    #29583

    Depositor

    Anna Huttenlocher

    Article

    Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

    Insert

    phosphatidylinositol phosphate kinase type I gamma
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Feb. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

    Plasmid

    #69907

    Purpose

    Expresses a circular RNA containing the 5' half of GFP in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    GFP, ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

    Plasmid

    #111459

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523), C643A

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. (363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

    Plasmid

    #168098

    Purpose

    Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    EPEA-tag, Flag-tag, and Ubc-Tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/cmv

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

    Plasmid

    #169992

    Purpose

    Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-siriusGFP-FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127316

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. XE254 pCDNA3.1(zeo)-hDsh3-497-716

    Plasmid

    #16755

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-497-716-Dsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa 497-716

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127318

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

    Plasmid

    #69894

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 FLAG mTOR 1968-2549 S2035T

    Plasmid

    #23000

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ()

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T aa 1968-2549 only

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

    Plasmid

    #62978

    Purpose

    Expresses myc-tagged mouse βTrCP residues 292-569

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codes for residues 292-569

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

    Plasmid

    #166796

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    KK93EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

    Plasmid

    #166800

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    E597R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

    Plasmid

    #49064

    Purpose

    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

    Plasmid

    #14443

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (1-461) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 1-461

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

    Plasmid

    #86818

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

    Plasmid

    #69896

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid

    #50888

    Purpose

    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NC12 pCDNA3.1 VENUS WTX delta ETGE

    Plasmid

    #36967

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (delta ETGE motif) (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    lacks amino acids 288 –291

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid

    #216491

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    NES, T2A-EGFP-CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge