We narrowed to 10,938 results for: AGA

1. pPpT4- TEF2prom-TIR1-FLAG

   Plasmid

   #189726

   Purpose

   Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   TEF2

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

   Plasmid

   #183387

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   redesigned Caspase-4 (AA94-377) (CASP4 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   T7

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-IRES-GFP-mouse-Caspase-1-H340D

   Plasmid

   #183362

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 H340D (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   H340D

   Promoter

   MESV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

   Plasmid

   #183364

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4a (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   H342D_K345M_K379T

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-IRES-GFP-dog-Caspase-1/4b

   Plasmid

   #183365

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4b (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-IRES-GFP-dog-Caspase-1/4a

   Plasmid

   #183363

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4a (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-Cas9

   Plasmid

   #120352

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   tet-on and CBh

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TAC-KRAB-dCas9

   Plasmid

   #120355

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   tet-on and CBh

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

   Plasmid

   #114730

   Purpose

   sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   sgRNA M7
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CeNL(Ca2+)_110μ-pcDNA3

    Plasmid

    #111929

    Purpose

    Cyan color bright luminescent calcium indicator with low affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRRNA-G11-R0

    Plasmid

    #101805

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Spy 1049

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-SsoPCNA3

    Plasmid

    #107069

    Purpose

    Expresses S. solfataricus PCNA3 fused to MBP in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Tags

    6xHis-thrombin cleavage site and MBP

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LEP-shTADA1.1580

    Plasmid

    #105568

    Purpose

    retrovirally express TADA1 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TADA1 shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    MSCV-LTR

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LEP-shTAF2.1635

    Plasmid

    #105561

    Purpose

    retrovirally express TAF2 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF2 shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    MSCV-LTR

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRRNA-G14-R0

    Plasmid

    #101806

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Spy 1049

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRRNA-G10-R0

    Plasmid

    #101804

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Spy 1049

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. BC-A1-016

    Plasmid

    #78704

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
    

    Use

    Synthetic Biology

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. BC-A1-015

    Plasmid

    #78703

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BC-A1-010

    Plasmid

    #78697

    Purpose

    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    

    Use

    Synthetic Biology

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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