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pEPMS0CM0024

Plasmid

#227510

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

CoTXSS (Calendula officinalis taraxasterol synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEPDG1CB0027

Plasmid

#227511

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

CaTXSS (Calendula arvensis taraxasterol synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEPMS0CM0033

Plasmid

#227512

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

CcTXSS (Cynara cardunculus taraxasterol synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEPMS0CM0036

Plasmid

#227513

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

HaTXSS (Helianthus annuus taraxasterol synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB027

Plasmid

#234636

Purpose

pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1102 (H16_RS24270 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB028

Plasmid

#234637

Purpose

pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1148 (H16_RS24495 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB029

Plasmid

#234638

Purpose

pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1264 (H16_RS25065 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB030

Plasmid

#234639

Purpose

pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1335 (H16_RS25400 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB024

Plasmid

#234633

Purpose

pET-28a(+) based plasmid for expression of H16_A0285 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A0285 (H16_RS01400 Cupriavidus necator H16)

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB026

Plasmid

#234635

Purpose

pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A3514 (H16_RS17530 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-mScarlet

Plasmid

#217453

Purpose

Gateway destination vector with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Type

Empty backbone

Use

Gateway destination vector

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-mScarlet-ORF

Plasmid

#217454

Purpose

Gateway destination vector with N-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Type

Empty backbone

Use

Gateway destination vector

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-YPet

Plasmid

#215541

Purpose

Expression vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-YPet

Plasmid

#215540

Purpose

Gateway entry vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia

Plasmid

#215442

Purpose

Gateway entry vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia

Plasmid

#215444

Purpose

Piggybac expression vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Transposon-based stable expression

Expression

Mammalian

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

Plasmid

#231568

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

All-in-one LOOMINA construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_dCas9-AsLOV2x3-NLSx3

Plasmid

#231567

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-BOLL

Plasmid

#222526

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BOLL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BOLL (BOLL Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KCTD19

Plasmid

#222529

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of KCTD19

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

KCTD19 (KCTD19 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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