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Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
UseTagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K L246D
Plasmid#112648PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K L246D
UseTagsExpressionYeastMutationR54K & L246D (in ALPS motif)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
UseTagsExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 Δ3xF AxxA
Plasmid#112650PurposeExpression S.cerevisiaeDepositorInsertGcs1 Δ3xF AxxA
UseTagsExpressionYeastMutationR54K, W304A, W307A, W310A, W349A, F353APromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
dRT-pMDLg/pRRE
Plasmid#60488Purposegag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)DepositorInsertHIV-1 gag, HIV-1 pol with inactive reverse transcriptase
UseTagsExpressionMutationchanged Asp at position 249 + 250 to ValPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
Plasmid#86424PurposePCR tagging module with yeast codon optimized mTurquoise2DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
UseTagskanMXExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-3HA
Plasmid#41608DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1
Plasmid#41605DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorInsertpCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1
Plasmid#41607DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GST
Plasmid#41611DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only