-
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK158_mCherry-4.1G-deltaCTD-hardened
Plasmid#46360DepositorInsert4.1G (EPB41L2 Human)
UseTagsmCherryExpressionMammalianMutationDeleted amino acids 886-1005, and modified for si…PromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MIS12-LAP-BUB1
Plasmid#115904PurposeUsed for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertMIS12-BUB1 (MIS12 Human)
UseTagsEYFPExpressionMammalianMutationBUB1 fused to MIS12PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/hTID1
Plasmid#13745DepositorInserthTid1 siRNA (DNAJA3 Human)
UseRNAiTagsExpressionMutationInverted repeat of 21 bp with 6 nuc. spacer.PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJBL7086
Plasmid#167250Purposeorthogonal Mango reporter output for cell-free expressionDepositorInsertpAKSIRV-Mango
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Plekhh1 5 silent mutations
Plasmid#187373PurposeExpresses human Plekhh1 with 5 silent mutations labelled with CherryDepositorInsertPlekhh1 with 5 silent mutations
UseTagsmCherryExpressionMammalianMutationFive silent mutations at the Plekhh1 siRNA site (…PromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0133v2
Plasmid#178395PurposeExpresses KSHV downstream MIR-SYNDepositorInsertKSHV downstream MIR-SYN
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112A and RNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F65A
Plasmid#65092PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange phenylalanine 65 to alanine (KDR backbone)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100A
Plasmid#65663PurposeFLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to alanine (KDR background)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F27A
Plasmid#65661PurposeFLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged phenylalanine 27 to alanine (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorInsertrecoded Homo sapiens PEG10 (PEG10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-V5-KDM3A
Plasmid#82331Purposelentiviral expression of KDM3ADepositorInsertKDM3A (KDM3A Human)
UseLentiviralTagsV5ExpressionMammalianMutationtwo silent mutations to make it siRNA resistantPromoterAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWMD23
Plasmid#140006PurposeExpression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)DepositorInsertsORF1 Shuffle 1 NLS
Pong TPase LA
UseTagsExpressionPlantMutationNES sequence mutated L418A, L420A and Ping N-term…PromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-AAV-sh[SNCA]
Plasmid#75437Purposeknockdown alpha-synuclein in rat neuronsDepositorInsertsnca (Snca Rat)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-LIR-mutant (LIRAAAA)
Plasmid#187981PurposePlasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationDDDW335-338AAAA (LIR mutant), silent mutation siR…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT3024
Plasmid#122542PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_mt::CemOrange2
UseTagsMLSLRQSIRFFKPATRTLCSSRTLLExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only