We narrowed to 4,114 results for: SPL

Showing: 1361 - 1380 of 4114 results

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1808_pmax-RBFOX1N-FRB-C

Plasmid

#118641

Purpose

Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-FRB-C

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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BAP1-010

Plasmid

#68366

Purpose

stable expression of BAP1 delta 221-238 in mammalian cells

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Parrotta et al J Thorac Oncol. 2017 Apr 4. pii: S1556-0

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Mutation

delta 221-238, splice variant found in human meso…

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
CMVTO-KRAS-G12V

Plasmid

#179654

Purpose

KRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12V

Expression

Mammalian

Mutation

G12V

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBFv-UAS3-GFP1-10

Plasmid

#138398

Purpose

Expresses split GFP fragment (GFP1-10)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

GFP1-10

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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V143 pLP-HA SD - Acceptor

Plasmid

#11710

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

HA

Expression

Mammalian

Available Since

June 22, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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SPDK3182

Plasmid

#97404

Purpose

binary construct of ER-targeted sfYFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfYFP1-10

Tags

ER target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3184

Plasmid

#97405

Purpose

binary construct of cytosolic sfCFP1-10 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfCFP1-10

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti sgRNA(MS2) mCherry

Plasmid

#171053

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

sgRNA(MS2) mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS956 IBB-GFP-mCherry3E

Plasmid

#107859

Purpose

Flow cytometry-compatible reporter for TDP43 function in splicing

Depositor

Rajat Rohatgi

Insert

Synthetic splicing reporter (TARDBP Synthetic)

Expression

Mammalian

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CTE-Luc

Plasmid

#60881

Purpose

Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

Luc-CTE

Expression

Mammalian

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMS-dsRed2-C1824U LMNA-GFP minigene

Plasmid

#128231

Purpose

C1824U mutation Lamin A splicing reporter

Depositor

Tom Misteli

Article

Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

Insert

lamin A (LMNA Human)

Tags

GFP and SV40-driven dsRed2 to identify transfecte…

Expression

Mammalian

Mutation

C1824U

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Fluc/CMV/WPREdU3

Plasmid

#136881

Purpose

Reporter vector expression firefly luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Firefly luciferase

Use

Lentiviral

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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P9_TEVs_cLuc

Plasmid

#119303

Purpose

Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cLuc connected to P9 coil via TEVs cleavable linker

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBHSTC104

Plasmid

#124356

Purpose

Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 N-intein and C-intein (bicistronic)

Tags

CcdA-C (SFAD)

Expression

Bacterial

Promoter

STC

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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