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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

Plasmid

#184645

Purpose

Retroviral expression of BirA Rap1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1-I312R

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215905

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe(LTR)-Cytochrome C-GFP

Plasmid

#41183

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pOZ-Pax7-FH-C-puro

Plasmid

#32518

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax7d (Pax7 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pICH41295(pro5U)-pAT2G29080(D,C)

Plasmid

#205370

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT2G29080(D,C)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly ZER1

Plasmid

#191540

Purpose

Protein expression of ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

ZER1 (ZER1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR C (709-1)

Plasmid

#17429

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pOZ-Pax3-FH-C-puro

Plasmid

#32517

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax3 (Pax3 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pmiRFP670-2-Actin-C-18

Plasmid

#197218

Purpose

Expresses the fusion protein of miRFP670-2-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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piRFP-FRB-PMP-C-10

Plasmid

#139316

Purpose

peroxisome-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP: MTOR(2021-2113):[GGSA]2QASNSAVSGLRSGSSGG:PXMP(2-195)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-C (w324-1)

Plasmid

#17404

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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TrmD_HaeIn/pET28b(+) N,C-tag

Plasmid

#12665

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 200

Insert

tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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CJ54 (cg5166a/c utr mut)

Plasmid

#17782

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg5166a/c utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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XE62 Xwnt-4 myc-SP64T

Plasmid

#16877

Depositor

Randall Moon

Insert

Xwnt-4 myc (wnt4 Frog)

Use

Xenopus expression

Tags

C-Myc

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
qTAG-C-mStayGold-EFS-Puro

Plasmid

#227256

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Puro

Plasmid

#227259

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Use

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-C-8His-Flag

Plasmid

#157678

Purpose

C-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

8His-Flag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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