We narrowed to 1,541 results for: halo

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pFsynW SYB2 reporter

Plasmid

#197276

Purpose

Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Use

Lentiviral

Tags

FLAG

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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px552-sg-gria1-HT-SEP

Plasmid

#187653

Purpose

Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

HaloTag-SEP donor

Use

AAV and CRISPR

Tags

N/A

Promoter

N/A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET51b-Strep-SsOGT-H5-His

Plasmid

#167277

Purpose

Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SsOGT-H5

Tags

His x10 and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-eSpCas9

Plasmid

#133370

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLz74_Rubisco_CsoS2_Npeptide_fusion

Plasmid

#140840

Purpose

Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS

Tags

CsoS2 N-peptide consensus to CbbL C-terminus and …

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pFUW-SNAP25B-D166Y

Plasmid

#248123

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with D166Y mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-D166Y (SNAP25 Human)

Expression

Mammalian

Mutation

D166Y

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-I192N

Plasmid

#248126

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with I192N mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-I192N (SNAP25 Human)

Expression

Mammalian

Mutation

I192N

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-K40E

Plasmid

#248127

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with K40E mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-K40E (SNAP25 Human)

Expression

Mammalian

Mutation

K40E

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-WT

Plasmid

#248121

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptosomal-Associated Protein (SNAP-25)

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B (SNAP25 Human)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-R59P

Plasmid

#248129

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with R59P mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-R59P (SNAP25 Human)

Expression

Mammalian

Mutation

R59P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-L50S

Plasmid

#248128

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with L50S mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-L50S (SNAP25 Human)

Expression

Mammalian

Mutation

L50S

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-A199G

Plasmid

#248122

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with A1999G mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-A199G (SNAP25 Human)

Expression

Mammalian

Mutation

A199G

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-G43R

Plasmid

#248124

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with G43R mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-G43R (SNAP25 Human)

Expression

Mammalian

Mutation

G43R

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-V48F

Plasmid

#248130

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with V48F mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-V48F (SNAP25 Human)

Expression

Mammalian

Mutation

V48F

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-I67N

Plasmid

#248125

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with I67N mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-I67N (SNAP25 Human)

Expression

Mammalian

Mutation

I67N

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

Plasmid

#236854

Purpose

Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

NRAS (NRAS Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFsynW SYT1deltaC2AB reporter

Plasmid

#197279

Purpose

Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)

Use

Lentiviral

Tags

FLAG

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-bhaB5

Plasmid

#167812

Purpose

Expresses His6- and MBP-tagged BhaB5 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB5

Tags

His6, MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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