We narrowed to 6,084 results for: nls

1. pGEM-T Easy-Cas9-nls-CDS1

   Plasmid

   #160231

   Purpose

   Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
   

   Use

   Level 0 of moclo golden gate position cds1

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

   Plasmid

   #186413

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli)
   

   Use

   Gateway destination vector

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ubc-nls-pcp-10xSunTag (SRv1.1)

   Plasmid

   #185798

   Purpose

   pcp-10xSunTag component for SunRISER SRv1.1

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   pcp-10xSunTag
   

   Use

   Tags

   FactorXa site, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ubc-nls-pcp-12xSunTag (SRv1.2)

   Plasmid

   #185799

   Purpose

   pcp-12xSunTag component for SunRISER SRv1.2

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   pcp-12xSunTag
   

   Use

   Tags

   FactorXa site, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-NLS-QPAS1-VP16

   Plasmid

   #186187

   Purpose

   AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

   Depositor

   Vladislav Verkhusha

   Article

   Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

   Insert

   NLS-QPAS1-VP16
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH321-1-Tier1-PhCMV-dCas9-3xNLS

   Plasmid

   #169595

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKSB- PUb-NLSmTurqSV40 GGGG AAGA

   Plasmid

   #174535

   Purpose

   PUb-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

   Depositor

   Eric Marois

   Insert

   PUb-mTurquoise BsaI cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti Polyubiquitin

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

   Plasmid

   #172208

   Purpose

   AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

   Plasmid

   #172205

   Purpose

   AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-PGK-dt/CBA-EGFP-NLS

    Plasmid

    #163918

    Purpose

    Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    dtomato and EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Added nuclear localization signal to EGFP

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaggs-NLS-PAmCherry1-GSS-EGFP

    Plasmid

    #158000

    Purpose

    Expression of nucleus localized PAmCherry1 and EGFP fusion protein

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    NLS-PAmCherry1-GSS-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-Synapsin-mTurquoise-3XNLS-WPRE

    Plasmid

    #159949

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise-3XNLS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Synapsin-Ypet-3XNLS-WPRE

    Plasmid

    #159950

    Purpose

    nucleus marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-3XNLS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-HOXD13(+7A)-IDR-YFP-NLS

    Plasmid

    #145278

    Purpose

    Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I

    Plasmid

    #130239

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-S5E7-dTom-nls-dTom

    Plasmid

    #135642

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E7 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-S5E9-dTom-nls-dTom

    Plasmid

    #135644

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E9 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

    Plasmid

    #133795

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a-3NLS-Klf4 TALE-GCN4

    Plasmid

    #120546

    Purpose

    Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ELK1 A box-NLS pcDNA3.1/HisC

    Plasmid

    #121096

    Purpose

    Expression of the A box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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