We narrowed to 1,583 results for: pbluescript

Showing: 1361 - 1380 of 1583 results

Results per page:

Sort results by:

HA tagged Sph ELL2 Met133, 138, 186 to Ileu

Plasmid

#127271

Purpose

For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu with HA tag after 8th Met

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met138ILeu ELL2

Plasmid

#127260

Purpose

For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met138 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met186ILeu ELL2

Plasmid

#127261

Purpose

For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met186 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met186 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met138ILeu, Met186ILeu ELL2

Plasmid

#127265

Purpose

For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (138,186) to ILeu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met133ILeu ELL2

Plasmid

#127259

Purpose

For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met133 to Ileu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met133ILeu, Met186ILeu ELL2

Plasmid

#127264

Purpose

For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (133,186 )to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met331Leu ELL2

Plasmid

#127262

Purpose

For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met331 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met331 to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met1ILeu ELL2

Plasmid

#127258

Purpose

For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met1 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met1 to Ileu by Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA tagged Xba ELL2

Plasmid

#127268

Purpose

For in vitro translation of WT ELL2 with HA tag inserted near N-term

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

Promoter

T7

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only
564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Vmn2r1-D-tauGFP TV

Plasmid

#108080

Purpose

Targeting vector for Vmn2r1 gene knockout mice (∆C1-GFP strain)

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r1-D-tauGFP-pA-ACNF (Vmn2r1 Mouse)

Use

Mouse Targeting

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV700

Plasmid

#119017

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

Plasmid

#122450

Purpose

Expression of inositol 1,4,5-trisphosphate 3-kinase A

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Tags

beta-galactosidase

Expression

Bacterial

Mutation

coding sequence from V66 till stop codon

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
rat brain inositol 1,4,5-trisphosphate 3-kinase A

Plasmid

#122155

Purpose

rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW1

Plasmid

#110220

Purpose

integration of loxP-ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV610

Plasmid

#119018

Purpose

RMCE vector containing inverted TP901-1 attB sites flanking large MCS

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attB sites

Use

Fruit fly rmce

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV609

Plasmid

#119014

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV688

Plasmid

#119015

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge