We narrowed to 1,479 results for: pbluescript
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Plasmid#234993PurposeExpression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvFLAG
Plasmid#234990PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA-3C-myc-scfvFLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvHA
Plasmid#234991PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinsDepositorInsertNA-3C-myc-scfvHA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
LB322 (Bcl-2 from ATG to -3934)
Plasmid#15381DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -3934 with both P1 and…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
PG13-luc (wt p53 binding sites)
Plasmid#16442PurposeLuciferase reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertp53-binding site (TP53 )
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
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INCTbiosyn-pUB-HspINTphiC31
Plasmid#127518PurposePlasmid encodes H. sapiens codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK321 (TOP2A-mAC (Neo))
Plasmid#140652PurposeTOP2A tagging with mAID-CloverDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B CA
Plasmid#49528Purposecloning vector for constitutively active chick BMP receptor 1BDepositorInsertBMPR-1B CA (BMPR1B Chicken)
UseTagsExpressionBacterialMutationconstitutively active mutation (Q203D); also P2A,…PromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-δWT-nAchR
Plasmid#97188PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertDelta nicotinic acetylcholine receptor (Chrnd Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-N
Plasmid#214734Purposeencodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoterDepositorInsertCAG_iPE-N_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-γWT-nAchR
Plasmid#97187PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertGamma nicotinic acetylcholine receptor (Chrng Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-C
Plasmid#214735Purposeencodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoterDepositorInsertCAG_iPE-C_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINTBxb1
Plasmid#127519PurposePlasmid encodes H. sapiens codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
aP2-GFPtpz
Plasmid#114211PurposeaP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophageDepositorInsertaP2 promoter + pOB4 + eGFPtopaz (Fabp4 Mouse, Synthetic, Aequorea victoria)
UseTagsNoneExpressionMammalianMutationPromoteraP2/Fabp4Available sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
UseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only