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We narrowed to 4,195 results for: spl

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Showing: 1361 - 1380 of 4195 results

  1. IpO

    Plasmid
    #48233
    Purpose
    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.
    Depositor
    Ronald Davis
    Publication
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    URA3 promoter and none
    Available since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48233-plasmid-map-sequence-id-69537

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  4. pAT9750-BEAR-mCherry

    Plasmid
    #162990
    Purpose
    BEAR target plasmid with split mCherry and disrupted 5' splice site
    Depositor
    Ervin Welker
    Publication
    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry split with an intron between amino acids 119-120
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162990-plasmid-map-sequence-id-324076

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  7. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162457-plasmid-map-sequence-id-321472

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  8. pDS-Red1-N1 AMPA GluR-B(flip)

    Plasmid
    #109142
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.
    Depositor
    Stefano Vicini
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPA receptor
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Glua2 subunit flip spliced form of the AMPA recep…
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109142-plasmid-map-sequence-id-209556

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  10. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

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  19. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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Showing: 1361 - 1380 of 4195 results