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PB-TO-hNIL BSD-mApple

Plasmid

#182313

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2 (NEUROG2 Human)
hISL1 (ISL1 Human)
hLHX3 (LHX3 Human)
bsd-T2A-mycNLS-mApple

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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AP1muA-Halo-ALFA-polyA-G418 HR

Plasmid

#229681

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Halo, ALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-5SA/S94A

Plasmid

#33103

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S61A, S94A, S109A, S127A, S164A, S381A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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Halo-Cyto

Plasmid

#124314

Purpose

Expresses cytosolic specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

Cytoplasm specific seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-EGFP

Plasmid

#247324

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez, Clotilde Thery

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

CD63 (CD63 Human)

Tags

SBP-EGFP (inserted in the small luminal loop of C…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDBB31

Plasmid

#248962

Purpose

DD-GAVPO (degron-regulated GAVPO) expression under control of the EF1alpha promoter in a PiggyBac integration plasmid

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Insert

DD-GAVPO

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-TeNT

Plasmid

#240990

Purpose

Expresses mCherry-TeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-TeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63YA-SBP-EGFP

Plasmid

#222327

Purpose

Synchronize the trafficking of CD63YA from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63-Y235A fused to SBP-EGFP (CD63 Human)

Expression

Mammalian

Mutation

Tyr235 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC022 (∆phcA)

Plasmid

#226200

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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