We narrowed to 128 results for: spycatcher

1. pAAV-P3-IgKL-SpyCatcher003-GINKO2

   Plasmid

   #234544

   Purpose

   to express a potassium sensor tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-GINKO2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   P3

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-P3-IgKL-SpyCatcher003-mNeonGreen

   Plasmid

   #234543

   Purpose

   to express a green fluorescent protein tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   P3

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-P3-IgKL-SpyCatcher003-SEP

   Plasmid

   #234541

   Purpose

   to express a pH sensor tagged with SpyCatcher in the liver

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   IgKL-SpyCatcher003-SEP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   P3

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET23a-His-TEV-SpyCatcher3

   Plasmid

   #235149

   Purpose

   Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   SpyCatcher3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-Cys-SpyCatcher (Min)

   Plasmid

   #216713

   Purpose

   Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

   Depositor

   Alex Dunn

   Article

   Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

   Insert

   Cys-SpyCatcher (Min)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See Li...Howarth, J. Mol. Biol., 2014

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMVTO-p450-hcvs-SpyCatcher

   Plasmid

   #179677

   Purpose

   HCVP-cleavable ER-associated SpyCatcher

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   HCVP SpyCatcher
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Use

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-SIN-BX SpyCatcher003-(short)mCD80

   Plasmid

   #210567

   Purpose

   Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-DIO-ECS-dSpyCatcher003

   Plasmid

   #234963

   Purpose

   Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   ECS-dSC
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

    Plasmid

    #234545

    Purpose

    to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

    Depositor

    Hajime Hirase

    Article

    Vittani et al bioRxiv 2025.03.18.643859

    Insert

    ECS-SC
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    mGFAP(ABC1D)

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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