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pGEX-parkin P37L

Plasmid

#227352

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin P37L (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Promoter

MSCV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154786

Purpose

Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

Depositor

Robert Singer

Article

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Fly, Synthetic)

Use

Lentiviral

Tags

GFP, V5, and mCherry

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-HA-mBec1 F121A

Plasmid

#99506

Purpose

Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

Depositor

Congcong He

Article

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Mutation

F121A

Promoter

CMV

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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AID-C12*-dCas9-BE4max

Plasmid

#216731

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-dCas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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AID-C12*-BE4max

Plasmid

#216729

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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R713-M50-303: CMV51p> Halotag-HRasHVR

Plasmid

#159685

Purpose

Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-HRasHVR (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M48-303: CMV51p> Halotag-Kras4aHVR

Plasmid

#159684

Purpose

Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4aHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R703-M80-303: CMV51p> Halotag-Kras4bHVR

Plasmid

#159683

Purpose

Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4bHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M52-303: CMV51p> Halotag-NRasHVR

Plasmid

#159686

Purpose

Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-NRasHVR (NRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRM200U

Plasmid

#44529

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pRM430

Plasmid

#44530

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-30

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pUK192

Plasmid

#44962

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

LEU2

Expression

Yeast

Mutation

HHTI-HHF1 is replaced with LEU2

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pRM214

Plasmid

#44527

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

K14R

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pRM204

Plasmid

#44528

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pRM415

Plasmid

#44526

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-15

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pRM102

Plasmid

#44525

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

P(GAL10)-HHT2 P(GAL1)-HHF2

Expression

Yeast

Promoter

GAL 10

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pET21a.MJ1594

Plasmid

#11413

Depositor

Sung-Hou Kim

Article

Wang et al Structure. 2001 Jan 10. 9(1):65-71.

Insert

phosphoserine phosphatase (MJ_1594 M. jannaschii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pMetR

Plasmid

#216329

Purpose

T7 promoter driving expression of metR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2023 Aug 18;12(8):2487-2

Insert

MetR

Expression

Bacterial

Available Since

Dec. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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