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We narrowed to 455 results for: CK1

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Nck

Plasmid

#186133

Purpose

For expression of recombinant Nck in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NCK1 (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acid residues 1-377, with mutations: C139S,…

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Blast

Plasmid

#123322

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Blast cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

ZsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProGL2 GW

Plasmid

#80689

Purpose

binary DEST vector for plant expression

Depositor

Arp Schnittger, Nico Dissmeyer

Article

Weinl et al Plant Cell. 2005 Jun;17(6):1704-22. Epub

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

ProGL2

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Tags

Expression

Mutation

Promoter

ADH1

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHYX137

Plasmid

#202814

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

ScPCK1

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2200

Plasmid

#170407

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->3] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 347-360)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2201

Plasmid

#170408

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->5] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 580-600)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2254

Plasmid

#170409

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->2] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2255

Plasmid

#170410

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->4] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ700-716, replace with 445-479)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2256

Plasmid

#170411

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->7] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 812-836)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2116

Plasmid

#170393

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 1 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ48-159)

Promoter

T7

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2117

Plasmid

#170394

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 2 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ160-279)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2118

Plasmid

#170395

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 3 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ280-391)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2119

Plasmid

#170396

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 4 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ392-515)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2120

Plasmid

#170397

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 5 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ516-633)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2121

Plasmid

#170398

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 6 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ634-746)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2122

Plasmid

#170399

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 7 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ747-877)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2199

Plasmid

#170406

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->1] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(Δ702-717, replace with 114-130)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1284

Plasmid

#175960

Purpose

For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Use

Tags

6xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2114

Plasmid

#175961

Purpose

For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Use

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ634-746

Promoter

araBAD

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pKB33 (pUC19-KANMX-tetO7-CYCpr)

Plasmid

#210472

Purpose

Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Insert

KanMX-TetO7-CYCpr cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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HeLa full-length MLCK-GFP

Plasmid

#46316

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase (MYLK Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

Plasmid

#210487

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge