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PaPchF-AMT

Plasmid

#242104

Purpose

Adenylase-methyltransferase didomain of the nonribosomal peptide synthetase Pchf from Pseudomonas aeruginosa for the production of pyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2019 Feb 12;58(6):665-678.

Insert

PaPchF-AMT

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

Plasmid

#244210

Purpose

Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFP

Plasmid

#244206

Purpose

Mammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastR

Plasmid

#244207

Purpose

Mammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastR

Plasmid

#244205

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

Plasmid

#244208

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

Plasmid

#244213

Purpose

Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM273_EF1A-dHyperLbCas12a-VPR-T2A-BlastR

Plasmid

#244211

Purpose

Mammalian expression of dHyperLbCas12a-VPR with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

Plasmid

#244216

Purpose

Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastR

Plasmid

#244215

Purpose

Mammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_pScaC(50-159)

Plasmid

#228836

Purpose

E. coli expression of a truncated ScaC passenger domain (aa 50-159)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 50-159

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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50_p5E_UBI_loxTagBFP

Plasmid

#172518

Purpose

p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision

Depositor

Jean Giacomotto

Article

Guo et al bioRxiv 2024.02.22.581357

Insert

ubiquitin promoter, TagBFP, loxP sites (inversion)

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSTAR2

Plasmid

#166940

Purpose

STAR-based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Small transcription activating RNAs

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSTAR

Plasmid

#166939

Purpose

STAR-based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Small Transcription activating RNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-03

Plasmid

#166934

Purpose

Target AD1.A6 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S6 - Block G- Flanked by BsaI sites

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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Rab33bb cds in pGem-T Easy

Plasmid

#80524

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab33bb

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRAJ22

Plasmid

#39251

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ22 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ22m

Plasmid

#39252

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ22 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ23

Plasmid

#39253

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ23 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ23m

Plasmid

#39254

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ23 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ31

Plasmid

#39255

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ31m

Plasmid

#39256

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-csChBP

Plasmid

#108876

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csChBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1c

Plasmid

#163682

Purpose

Far-red fluorescent calcium indicator FR-GECO1c

Depositor

Robert Campbell

Article

Dalangin et al bioRxiv 2020.11.12.380089

Insert

FR-GECO1c

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1a

Plasmid

#163681

Purpose

Far-red fluorescent calcium indicator FR-GECO1a

Depositor

Robert Campbell

Article

Dalangin et al bioRxiv 2020.11.12.380089

Insert

FR-GECO1a

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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