Skip to main content
Addgene
Showing: 121 - 140 of 653 results

  1. pTU1-C-cat

    Plasmid
    #112791
    Purpose
    Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112791-plasmid-map-sequence-id-218393

    Enlarge

  2. pTU1-D-rrnOD

    Plasmid
    #112786
    Purpose
    Downstream homology arm, rrnO-23S (23S rRNA)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    rrnOD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112786-plasmid-map-sequence-id-218378

    Enlarge

  3. pTU1-D-amyED

    Plasmid
    #112782
    Purpose
    Downstream homology arm, amyE encodes _-amylase
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    amyED
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112782-plasmid-map-sequence-id-218373

    Enlarge

  4. pBsVchCAST-pyrE

    Plasmid
    #203813
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203813-plasmid-map-sequence-id-403827

    Enlarge

  5. pBsVchCAST

    Plasmid
    #203812
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203812-plasmid-map-sequence-id-403828

    Enlarge

  6. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

    Enlarge

  7. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

    Enlarge

  8. pIA028

    Plasmid
    #200419
    Purpose
    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    PAmCherry1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200419-plasmid-map-sequence-id-405382

    Enlarge

  9. pIA033

    Plasmid
    #200420
    Purpose
    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsPAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200420-plasmid-map-sequence-id-403933

    Enlarge

  10. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200421-plasmid-map-sequence-id-405458

    Enlarge

  11. pBP-ORF-xylE

    Plasmid
    #112780
    Purpose
    Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    xylE open reading frame
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112780-plasmid-map-sequence-id-218368

    Enlarge

  13. pTU1-D-recJD

    Plasmid
    #112784
    Purpose
    Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    recJD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112784-plasmid-map-sequence-id-218375

    Enlarge

  14. pTU1-A-yckABU

    Plasmid
    #112789
    Purpose
    Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    yckABU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112789-plasmid-map-sequence-id-218385

    Enlarge

  15. pBP-PliaG

    Plasmid
    #112777
    Purpose
    Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    PliaG promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112777-plasmid-map-sequence-id-218358

    Enlarge

  17. pTU2-AmyE-Pveg-eGFP

    Plasmid
    #112792
    Purpose
    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg-eGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112792-plasmid-map-sequence-id-218394

    Enlarge

  18. pTU1-D-yckABD

    Plasmid
    #112790
    Purpose
    Downstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    yckABD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112790-plasmid-map-sequence-id-218386

    Enlarge

Showing: 121 - 140 of 653 results