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pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
UseTagsExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Sc
Plasmid#109365PurposeHuman rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tagDepositorInsertUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Am
Plasmid#109364PurposeHuman rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tagDepositorInsertRod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Human, Branchiostoma belcheri)
UseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
UseTagsmCherryExpressionMammalianMutationPromoterLTRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT I261A
Plasmid#153476PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT I261ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, I261APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT S333A
Plasmid#153477PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT S333ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, S333APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta2step
Plasmid#153479PurposeMammalian expression of FLAG-tagged Tapasin delta2stepDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationdeletion of aa.13-14, 16-17PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta3step
Plasmid#153480PurposeMammalian expression of FLAG-tagged Tapasin delta3stepDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationdeletion of aa.12-14, 16-18PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15E
Plasmid#153481PurposeMammalian expression of FLAG-tagged Tapasin G15EDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationG15EPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15L
Plasmid#153482PurposeMammalian expression of FLAG-tagged Tapasin G15LDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationG15LPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18E
Plasmid#153483PurposeMammalian expression of FLAG-tagged Tapasin L18EDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18EPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18G
Plasmid#153484PurposeMammalian expression of FLAG-tagged Tapasin L18GDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18GPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only
