Addgene

We narrowed to 140 results for: akt1

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Human, Budding Yeast, Synthetic)

Use

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt-AH(R25C)

Plasmid

#39534

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (PH domain only) (Akt1 Mouse)

Use

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

PH domain only with R25C mutation

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-HA-YFP-PRAS40

Plasmid

#73389

Purpose

Expresses HA and YFP tagged PRAS40

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

AKT1 Substrate 1 (Proline-Rich) (AKT1S1 Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
PH Akt-Venus

Plasmid

#85223

Purpose

Venus tagged PH domain of Akt for use as a PIP3 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

PH AKT(1-148) (AKT1 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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GAG-PKB-pcDNA3

Plasmid

#24651

Depositor

Jim Woodgett

Article

Jones et al J Exp Med. 2000 May 15. 191(10):1721-34.

Insert

PKB (AKT1 Human)

Use

Tags

GAG

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pSi-check2-hRac2 3'UTR

Plasmid

#97160

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

RAC 3'UTR (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pHRIG-PRAS40

Plasmid

#53599

Purpose

lentiviral expression of constitutively active PRAS40 and EGFP

Depositor

Heng Zhao

Article

Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

Insert

PRAS40 (AKT1S1 Human)

Use

Lentiviral

Tags

IRES-EGFP and myc

Expression

Mammalian

Mutation

constitutively active (myristoylated version)

Promoter

CMV

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pSi-check2-hRac2 - mut 3'UTR

Plasmid

#97161

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

RAC 3'UTR (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

miR-722 Binding Mutant

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag PRAS40

Plasmid

#14950

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-HA-PRAS40

Plasmid

#15481

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PRAS40

Plasmid

#15476

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-HA-PRAS40 T246A

Plasmid

#15482

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Thr246 to Ala

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1-PRAS40

Plasmid

#15477

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (AKT1S1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-myc-PRAS40 T246A

Plasmid

#15479

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Thr246 to Ala

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1-mPRAS40

Plasmid

#15480

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (Akt1s1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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HA-PRAS40 WT

Plasmid

#86758

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-PRAS40 AA

Plasmid

#86759

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

AA (S183A, S221A)

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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HA-PRAS40 F129A

Plasmid

#86763

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F129A

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-PRAS40 W10A

Plasmid

#86761

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

W10A

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge